Identification of the prolactin cDNA sequence in Atlantic herring (Clupea harengus)

Jens Thaulow Pedersen & Kristian Meier

Studenteropgave: Semesterprojekt

Abstrakt

Prolactin is a protein hormone involved in osmoregulation in teleost species. The Atlantic herring (Clupea harengus) is a marine teleost which is found in environments of different salinities. The local herring population’s possible use of spawning areas with different salinities could involve a difference in genetic selection. The aim of this project was to determine the prolactin cDNA sequence in Atlantic herring in order to look at the expression of this protein hormone. Individuals were sampled from two locations with different salinities, possibly representing two populations, in order to establish if any differences could be found at the protein hormone level. At present a partial cDNA sequence for prolactin has been obtained. The sequence consists of 501 nucleotides and codes for 167 amino acids. The 5’end and 3’end as well as a small gap of 21 nucleotides still needs to be sequenced. Also the sequence still needs to be obtained from individuals from both locations in order to compare the cDNA. Despite the missing parts identities between 59-73% was found when comparing with 19 other teleost species.

UddannelserMolekylærbiologi, (Bachelor/kandidatuddannelse) Bachelor el. kandidat
SprogEngelsk
Udgivelsesdato1 jun. 2006
VejledereTove Atlung

Emneord

  • Herring
  • Prolactin
  • Osmoregulation