The purpose of this project is to develop an experiment in relation to a scientific problem. This project is based on a biological experiment that examines the relation between the HOXA9-gene, which is a regulatory gene during embryogenesis, and the transcription factor CDX2. Studies have shown that there is an overexpression of HOXA9 and CDX2 in people suffering from leukemia, especially acute myeloid leukemia and acute lymphoblastic leuke-mia. Research has also shown that CDX2 is able to bind to the promoter for HOXA9. This project will examine the connection between the expression of HOXA9 and CDX2 in relation to leukemia. This is done by gathering knowledge on topics such as HOX-genes, CDX2 and leukemia while developing two different experiments. One experiment deals with the promot-er for HOXA9 and CDX2 and the other deals with the actual HOXA9-gene. By creating a primer and thus extracting the DNA-sequence of interest, the desirable sequence will be put into separate plasmids using in-fusion cloning. The cloned plasmids will be put into mammal cells to look at the sequence’s expression. The HOXA9-promoter is exposed to CDX2 and the expression is then measured through luciferase, which the promoter is placed in front of. For the other experiment dealing with the HOXA9-gene, the purpose is to measure the expression of HOXA9 after they have been placed in human cells, to see if it is overexpressed on its own. These results can then be compared to the expression in the other experiment, but unfortu-nately the last part was unsuccessful and there are no results from that experiment. For the experiment with the HOXA9-promoter, there is an obvious overexpression of HOXA9 when it has been exposed to CDX2 and it is consequently possible to conclude that the expression of HOXA9 is influenced by CDX2. The relation to leukemia is still unknown, but interesting for further research.
|Uddannelser||Basis - Naturvidenskabelig Bacheloruddannelse, (Bachelor uddannelse) Basis|
|Udgivelsesdato||19 jun. 2013|