Hemolysin-producing Escherichia coli in inflammatory bowel disease patients

Maiada El-Khatib, Simone Hogrefe Holm, Knud Erik Adam Geraae, Charlotte Jørgensen, Dana Backran & Vithusiga Karunanthan

Studenteropgave: Fagmodulprojekt

Abstrakt

This project revolves around how to detect alpha-hemolysin in Escherichia coli and how the alpha- hemolysin affects the intestines. Throughout the report we investigate the function of alpha-hemolysin, the inflammatory bowel disease with a focus on Ulcerative colitis. In addition, we investigate PCR-method, gel electrophoresis and how to detect alpha-hemolysin on horse- and sheep blood plates.
We have made some experiments to detect the alpha-hemolysin in Escherichia coli from patient samples with and without Ulcerative colitis. These results showed that two out of six Escherichia coli strains from two Ulcerative colitis patients had a positive gene for alpha-hemolysin. In addition, we had three negative strains from three Ulcerative colitis patients and one healthy person with a negative gene for alpha-hemolysin. One of the Ulcerative colitis patients had two strains one positive and one negative for alpha-hemolysin.
In this study we discuss, which methods are the best to detect the alpha-hemolysin and if the alpha-hemolysin is the cause or the enhancing factor of the permeability in the intestines in patients with Ulcerative colitis.

UddannelserBasis - Naturvidenskabelig Bacheloruddannelse, (Bachelor uddannelse) Bachelor
SprogEngelsk
Udgivelsesdato17 dec. 2019
Antal sider51
VejledereKaren Angeliki Krogfelt & Hengameh Chloé Lauridsen

Emneord

  • Eshcerichia coli
  • E. coli
  • Inflammatory bowel disease
  • IBD
  • Hemolysin
  • Alpha-hemolysin
  • Ulcerative colitis