608,000 people died worldwide in 2008 due to colorectal carcinoma, which makes cancer research essential. In recent years, research within epigenetics has become of growing importance to carcinogenesis, therefore epigenetic modifications are now accepted on same terms as genetic changes. Epigenetic modifications are of great importance in regulation of oncogenes and tumor suppressor genes, partly through DNA methylation. Furthermore, the reversibility of epigenetic modifications can provide new possibilities in future cancer treatment. In this project gene expression of the following DNA methyltransferases; DNMT1, DNMT3a and DNMT3b, and of the methyl binding proteins; MBD2, MeCP2 and Kaiso, was investigated in the colon cancer cell lines; SW480, DLD-1 and HCT116. This was tested experimentally with Hot Start PCR and agarose gel electrophoresis. We found gene expression of DNMT1, DNMT3b, MBD2 and MeCP2 in all three colon cancer cell lines. Gene expression of Kaiso was found in SW480 and DLD-1. Gene expression of DNMT3a could not be determined in the investigated colon cancer cell lines.
|Uddannelser||Molekylærbiologi, (Bachelor/kandidatuddannelse) Bachelor el. kandidatAlmen Biologi, (Bachelor/kandidatuddannelse) Bachelor el. kandidat|
|Udgivelsesdato||24 jan. 2014|
- DNA methylering