Fundamental study of recombination and recombineering in Escherichia coli

Xiaohang Sun & Yang Huang

Studenteropgave: Semesterprojekt

Abstrakt

Recombination and recombineering systems have been used in Escherichia coli to recombinant DNA sequences. With endonuclease and DNA lipase the bacterial plasmid and target DNA fragment can bind together and recombinant for a new DNA sequences. Red Proteins have been used in recombineering system to perform the function as the enzymes in recombination system, and faster and easier than the other way of recombinant new DNA sequences in E.coli. In this report we get to know the principles of recombination and recombineering, and how to perform the experiments. We also discussed the differences between these two technologies. Sooner this system will be important used in more widely areas such as molecular biology, medical biology and etc.

UddannelserMolekylærbiologi, (Bachelor/kandidatuddannelse) Bachelor el. kandidat
SprogEngelsk
Udgivelsesdato8 jan. 2008
VejledereOle Skovgaard

Emneord

  • recombination
  • RecA
  • Red/ET
  • recombineering