In the present project, we studied the subcellular localization of the yidD gene product 9K by making the fusion proteins GFP-9K and 9K-GFP (GFP: Green Fluorescent Protein). In order to study protein-protein interaction between 9K and possible soluble protein, we fused yidD to gst (Glutathione-S-transferase) and did a pull down assay. Further, we tested the toxicity of 9K by expressing it at different level. Growth experiments were also done with a wild-type strain and a knock-out strain with Tn10 inserted into yidD. We found out that 9K seems to be localizes to the inner membrane of the cell due to some physically interactions between its C-terminus and some membrane protein. Both over expression and the lack of 9K seem to affect cell growth and the lack of 9K seems to interfere with replication of the DNA, in some unknown fashion.
|Uddannelser||Molekylærbiologi, (Bachelor/kandidatuddannelse) Bachelor el. kandidat|
|Udgivelsesdato||1 jun. 2006|
- flow cytometry