Cancer is the leading cause of death worldwide and CRC is the third most common type. Many factors contribute to tumorigenesis including different types of epigenetic alterations of cancer-critical genes as seen in tumor suppressor genes and oncogenes. For example genes situated in the NDRG family have been demonstrated to contain tumor suppressor effects. The NDRG family has been studied in numerous cancer types, but these studies have given different conclusions in regard to the function. In this thesis the role of the NDRG family has been studied in four different CRC cancer cell lines to investigate whether the promoter region of the genes were methylated, and thus epigenetically silenced. Studies have shown that MBPs bind to methylated CpG islands and afterwards recruit transferases, which leads to the formation of heterochromatin. By isolating RNA from cell lines the thesis showed that the MBPs MeCP2, Kaiso, and MBD2 were present at mRNA and protein level. ChIP followed by qPCR confirmed the interaction between MBPs and NDRG4 chromatin. Lastly, RNAi was performed with esiRNA to see if it was able to knockdown the MBPs and thereby reactivate the NDRG genes. No definitive conclusion could be drawn, due to contradictory results. However, this study demonstrates that NDRG1 is not methylated, while NDRG4 is methylated and interacts with the MBPs MeCP2 and MBD2.
|Uddannelser||Molekylærbiologi, (Bachelor/kandidatuddannelse) KandidatMedicinalbiologi, (Bachelor/kandidatuddannelse) Kandidat|
|Udgivelsesdato||3 aug. 2015|
- NDRG family