Effects of 4-n-nonylphenol on the Prosobranch Snail Marisa cornuarietis

Mads-Peter Jakob Dahl, Nadja M Mariager & Sandra F Hansen

Studenteropgave: Semesterprojekt


The awareness of endocrine disrupting chemicals (EDC’s) has increased throughout the last decade due to great amounts released into the environment by the industry. In this study we have investigated the effect of the abundant EDC, 4-n-nonylphenol, on the gastropod Marisa cornuarietis, as very little attention has been dedicated to the invertebrates responses to EDC’s. M. cornuarietis was exposed to a nominal concentration of 4-n-NP of 100µg/L for a period of 34 days. The experiment was performed as a static renewal test ensuring that the snails were continuously exposed to 4-n-NP. During the experiment eggs were collected and counted three times a week to investigate 4-n-NP’s effect on the fecundity. Water samples from exposed aquaria were taken several times a week to measure the exact concentration of 4-n-NP as it was expected that the concentration would decrease over time. After three weeks clutches of eggs from exposed and unexposed snails were set to hatch for the purpose of investigating the hatchability success of the two types of eggs. Furthermore the concentration of 4-n-NP in the exposed snails was measured as well as their content of lipid. Our results showed no significantly difference in fecundity between the exposed and unexposed snails. A significantly higher hatchability success of the eggs from unexposed compared to exposed snails after eight days of hatching was detected. A relatively low content of 4-n-NP, approximately 10ng/g, were found in eight exposed snails after 34 days of exposure to 4-n-NP which conflicts with the high content of lipid, approximately 30%, found in the same eight snails. Uncertainties in the extraction procedure made concentration results from extraction of water samples and snails unreliable.

UddannelserMolekylærbiologi, (Bachelor/kandidatuddannelse) Kandidat
Udgivelsesdato1 jun. 2005
VejledereAnnemette Palmqvist


  • GC-MS
  • Marisa cornuarietis
  • prosobranchs
  • 4-n-nonylphenol
  • endocrine disruptors