DNA sequencing and short reads assembly

Gang Chen

Studenteropgave: Semesterprojekt

Abstrakt

Next-generation DNA sequencing platforms have become widely available, reducing the cost of DNA sequencing and increasing data throughput of DNA sequence. These new technologies promise a new ear in the applications of DNA sequence such as gene expression, cancer genome research, ChIP-Seq and RNA-Seq. However, some of them produce very short reads, typically tens of base pairs, which demands new algorithms and software to generate entire DNA sequence. The difficulty of short reads assembly is from high error probability of reads alignment and ambiguity within the genome. This report describes the principle of DNA sequencing and some applications of sequence information, discusses two reference assembly softwares, MAQ and BOWTIE.

UddannelserAlmen Biologi, (Bachelor/kandidatuddannelse) Bachelor el. kandidat
SprogEngelsk
Udgivelsesdato21 jan. 2009
VejledereOle Skovgaard

Emneord

  • short reads assembly
  • DNA sequencing