Cytochrome P450 Enzymatic Response to Fluoranthene in the Polychaete Alitta virens

Balder Nehring Madsen, Jacob Martin Nielsen, Louise Jøhnk Stabell, Martine Pedersen Lynderup & Jonathan Yngve Bech Jedal

Studenteropgave: Fagmodulprojekt


Polycyclic aromatic hydrocarbons are a group of highly toxic compounds that are widely
distributed in the environment, mainly by anthropogenic sources. Cytochrome P450 proteins, found in both vertebrates and invertebrates, including polychaetes, can metabolize these polyaromatic hydrocarbons through mixed function oxidases. Two cytochrome P450 genes have been isolated from the polychaete Alitta virens, which has shown the ability to biotransform xenobiotics such as polycyclic aromatic hydrocarbons, but the details of the underlying mechanism of the gene expression remains unclear. In this experiment, 40 A. virens were exposed to the polycyclic aromatic hydrocarbon fluoranthene in three different concentrations, 15 μg, 30 μg and 60 μg per g of dry-weight sediment. To elucidate any possible microbial effects on
cytochrome P450 activity, 20 of the specimens were treated with antibiotics. An ECOD fluorometric assay was conducted to investigate mixed function oxygenase activity within A. virens gut microsomes. Results showed no ECOD activity, neither did the antibiotics seem to have any noticeable effect.

UddannelserBasis - Naturvidenskabelig Bacheloruddannelse, (Bachelor uddannelse) Basis
Udgivelsesdato5 dec. 2017
Antal sider9