Comet assay on the anhydrobiotic Richtersius coronifer

Johannes Lørup Johnsen, Andreas Møller-Nybye, Allen Njimeri Nyendi & Michelle Schollert Skovgaard

Studenteropgave: Semesterprojekt

Abstrakt

The tardigrade, Richtersius coronifer has an ability to withstand desiccation by going into anhydrobiosis. During anhydrobisis, metabolism is arrested, which leads to an accumulation of DNA damage, since repair systems are inactive. The understanding of desiccation tolerance in tardigrades is still superficial, and it is rather unclear if desiccation tolerance in tardigrades is due to an effective repair system or if it is connected to an efficient protection of DNA and other cell components. The repair system of R. coronifer upon rehydration is investigated by using comet assay to detect DNA damages in animals at 0 and 24 hours after rehydration. There was found no evidence for an efficient repair system in R. coronifer. Still, it seems likely that R. coronifer possesses a complex combination of adaptations, including a considerable repair system, to exhibit successful anhydrobiosis and revival. Inevitably, more investigations must be done to demonstrate this claim.

UddannelserMiljøbiologi, (Bachelor/kandidatuddannelse) Bachelor el. kandidatMolekylærbiologi, (Bachelor/kandidatuddannelse) Bachelor el. kandidat
SprogEngelsk
Udgivelsesdato1 jun. 2009
VejledereHans Ramløv

Emneord

  • cryptobiosis
  • comet assay
  • tardigrade
  • richtersius coronifer