Relationship between cancer and environmental factors were discussed in recent studies. Based on epidemioloically and experimental studies in experimental animals it is clear that diet components may play an important role in cancer prevention. It’s reason cell cycle is related with not only internal signals but also external signals which include dietary compounds‘ deriatives. The cruciferous plant family is playing an outstanding role in cancer prevention and has being investigated in a lot of studies from past to present. Cruciferous vegetables contain phytochemicals. They have some potential affect on cell cycle. This family has various indoles including indole-3-ylcarbinol (I3C) and N-methoxyindole3-ylcarbinol (NI3C) . Up to now the biological activities of these indoles have been estimated only when exposed to the single indole, whereas we here analyse the combinatory effect of I3C and NI3C together inhibiting the cell proliferation of HCT 116 colon epithelial cancer cell line. So aim of this study is to estimate the inhibitory effect of indole-3-ylcarbinol or N-methoxyindole-3-ylcarbinol on cell proliferation and to evaluate the combinatory effect when exposing the I3C and NI3C together in HCT 116 cell line . Limits of project are stated like determining these compounds impact on cell proliferation in vitro with HCT 116 cell line. The cells were tested for 1,9 – 500 µM I3C and 5-100 µM NI3C alone and in combination for 24, 48 and 72 hours. Although decreasing cell number is observed in single treatment of NI3C on time and dose-dependent affect, I3C single treatment did not show any meaningful response and IC50 values for I3C was determined at highest concentrations like 500 µM after 24 hours treatment. Concentraton of I3C was selected 415 µM for IC50 after reviewed all time point results and for NI3C is determined 70 µM. When two compounds were combined, more cells were influenced with combinatory affect than single usage. However When the compunds ratios is 1:2, no sensible response could be get, on the other side results were sensible in 1:1 ratio . 72 hours results in co-treatment effected highest cell viability so this shows that there is existence of combinatory affect dependent to time for these compounds. In conclusion, a combinatory effect of I3C and NI3C on cell proliferation was observed, which may explain the effect cancer preventive effect of cruciferous vegetables, where I3C and NI3C is found in combination.
|Uddannelser||Molekylærbiologi, (Bachelor/kandidatuddannelse) Bachelor el. kandidat|
|Udgivelsesdato||8 jun. 2011|
- indoles, I3C, NI3C,