Characterization of the pmt protein complex from Staphylococcus aureus

Lykke Breum Madsen, Peter Rejic Puhar, Michael Masciotti, Nur Konyalilar, Irmak Kaysudu & Niamh Lacy-Roberts

Studenteropgave: Fagmodulprojekt

Abstrakt

Antibiotics are the main treatment against bacterial infections and illnesses, since Alexander Fleming discovered penicillin in 1928. Staphylococcus aureus (S. aureus) is a Gram positive bacterium which causes variety of diseases due to its receptive abilities to substrates, which in tun contributes to its antibiotic resistance. During infections PSMs (phenol soluble modulins) are secreted by PMT (phenol soluble modulin transporter), which play a key role in S. aureus virulence. In the experiment the PSM exporter of Staphylococcus aureus was investigated. The aim of this project was the characterization of the 3D structure of PmtA and PmtC proteins of Staphylococcus aureus, so it can be used in drug development against the diseases that Staphylococcus aureus causes. To accomplish this aim, firstly, the transformation of pmtA and pmtC loci from Staphylococcus aureus to E.coli was done. After the cell cultures grew, proteins that were expressed from these loci were purified from the bacteria. Lastly, the proteins should have been concentrated in order to complete the crystallization, however this was not successful. The final step of the project, protein crystallization, could not be attained, since the desired proteins could not be obtained as concentrated as needed. According to the trials it was understood that pmtA and pmtC loci need to be on the same plasmid while making the transformation to gain proper folded proteins.

UddannelserMolekylærbiologi, (Bachelor/kandidatuddannelse) Bachelor
SprogEngelsk
Udgivelsesdato29 maj 2017
Antal sider42
VejledereKornelius Zeth

Emneord

  • Protein, PSM, ABC transporter, antibiotics, Staphylococcus Aureus