CDX2 is a gene, which codes for a transcription factor: CDX2A. This gene has a crucial role in expression of specific genes in intestinal epithelial cells and differentiation and migration of the cells. Studies show that transscription of the CDX2 gene is decreased in patients with Inflammatory Bowel Disease (IBD). That causes the intestinal epithelial cells to not differentiate sufficiently. Other studies show that the CDX2 gene also expresses a variant of the protein CDX2A; vCDX2C. The vCDX2C-mRNA is up regulated in patients with IBD, which indicates that the vCDX2C protein reduces the CDX2A proteins function. We have examined whether or not the vCDX2C protein is dominant negative to the CDX2A protein through promoter analysis with two different promoters: LPH and SI in HeLa cells. We saw that both the vCDX2C protein and the CDX2A protein reduced the LPH promoters activity with about 20% in HeLa cells. Together the tro proteins reduced the LPH promoters activity with about 40% in HeLa cells. Furthermore, the promoter studies showed that the presents of the CDX2A protein increased the SI promoters activity with about 50% in HeLa cells. The presents of the vCDX2C protein decreased the SI promoters activity with about 10% in HeLa cells. In the presents of both proteins the vCDX2C protein decreased the CDX2A proteins increasing effect with more than 30% i HeLa cells. We found that the vCDX2C protein was dominant negative to the CDX2A protein for the SI promoter in HeLa cells.
|Uddannelser||Basis - Naturvidenskabelig Bacheloruddannelse, (Bachelor uddannelse) Basis|
|Udgivelsesdato||3 jun. 2014|