Enteroaggregative Escherichia coli (EAEC) is a major cause of disease throughout the world. An important virulence factor for this type of E. coli is the aggregative adherence fimbriae (AAF) of which several variants exist. The binding sites for these fimbriae are though yet to be discovered. This report will study the severity of blood infections caused by the AAF/V-producing EAEC strain C338-14, by analysing the adhesion to blood types within the AB0 and Rhesus-system (hemaagglutination). The method of hemaagglutination is used to detect the presence of adhesins. This was done by visualisation of the hemaagglutination as well quantification of the amount of erythrocytes and bacteria. The data produced has given an understanding of the difference in EAEC adhesion and clearly shows that some blood types, especially those including B-antigens, are more prone to adhesion than others. Moreover, the experiments have shown sialic acid to be a possible binding site for AAF/V to adhere to the antigens of erythrocytes. This information can be used to improve the treatment of patients with EAEC blood infections and give insight into other types of bacterial blood infections.
|Uddannelser||Molekylærbiologi, (Bachelor/kandidatuddannelse) Bachelor|
|Udgivelsesdato||31 maj 2016|
- Enteroaggregative Escherichia coli (EAEC)