#This thesis encompasses two parts. The first part deals with general introduction to isolation, characterization, and bioactivities of natural products, with emphasis on Jasminum Subtriplinerve Blume spp., and its genus. The second is experimental study of J.subtriplinerve Bl. about its bioactivities and chemical constituents. In first part (from chapter 1 to 6), the botanical of two varieties of J.subtriplinerve Bl., a medicinal plant widely distributed in Middle area of Vietnam that has just been paid attention, was firstly described and distinguished. In addition, the bioactivities and constituents studies on some species of this genus are also focus on as well as the analytical techniques and methods of researching. The remained parts presents the experiments of methods used in investigation of bioactivities, isolation, and structural elucidation of J.subtriplinerve Bl var. Vang Se followed by a synopsis of results and discussion. The in vitro bioactive tests of extracts from two extraction methods include antimicrobial assay of four bacteria, two fungi and two yeasts; antioxidant activity by DPPH (2,2-diphenyl-picryhydrazyl) radical scavenging method; and in cytotoxic test against four human cancerous cell lines: Hep-2 (human hepatocellular carcinoma), RD (human heart membrane), LU (human cervical cancer) and DLD-1 (human colon cancer). The isolation of petroleum and ethyl acetate extracts are given three common compounds, 3β-acetyl oleanolic acid, Lup–20–en–3β–ol, and Stigmast-5-en-3-O-β-ol. However, the cytotoxic test brought about attracted results that attested the petroleum ether and chloroform extract having activities. Among the extracts, which had test the cytotoxic activity, S.A1-1 exhibited most potential activity by reducing the percentage of cell survival by 95% at concentration of 25μg/ml after 48hr treatment, especially when increasing its concentration 10 times, this value dropped by 3%.
|Uddannelser||Kemi, (Bachelor/kandidatuddannelse) Bachelor el. kandidat|
|Udgivelsesdato||1 jun. 2006|
|Vejledere||Poul Erik Hansen & Ole Vang|