Anvendeligheden af mikrokalorimetri til at måle stress inducerede metabolismeændringer hos Hyalella azteca (orden: amphipoda)

Jeppe H Floor

Studenteropgave: Speciale

Abstrakt

Most metabolic measures of aquatic invertebrates are carried out by indirect measures of the O2 uptake or CO2 production. But intake and production of these gases respectively are highly dependent on the substrate being decomposed. Direct heat can provide a quantitative expression for the metabolism, since this is equal to the heat emitted and the mechanical work performed. Microcalorimetry is a method traditionally designed to measure the heat of polymers and medicinal substances, and this study aims to investigate whether this method can be applied to living aquatic organisms. Measurements were made on the amphipod Hyalella azteca (sassure) on a differential scanning calorimeter. The organism was exposed to 6 mg/L of organophosphate Dimethoat. Measurements of water alone was found to be problematic for short periods of time (1.5 hours), but these were more stable over longer measurements (20 hours). It was found that there are statistical evidence to measure the organisms (p = 0.000), and that stress-induced changes in metabolism could also be measured (p = 0.001). A very large scatter between measurements generally means, however, that this method is very questionable and no findings in this study indicate that microcalorimetry is currently a useful method to use on a routine basis

UddannelserMiljøbiologi, (Bachelor/kandidatuddannelse) Kandidat
SprogDansk
Udgivelsesdato10 mar. 2014
VejledereAnnemette Palmqvist

Emneord

  • H. azteca
  • Organofosfat
  • Mikrokalorimetri
  • Metabolisme
  • Dimethoat