Antimicrobial Resistance

Jan Radic, Ernesto Calderon, Marta Stenz, Kristian Sørensen & Sibghat Ullah

Studenteropgave: Semesterprojekt

Abstrakt

Antimicrobial resistance has been increasing alongside with antibiotic use, and with less new antibiotics being produced it is imperative to find new ways to treat bacterial infections. The causes of resistance are numerous. Although it is not completely clear how the resistant genes are created, it is known that the selective pressure created due to anthropogenic activities has led to the creation of environmental reservoirs of antibiotic resistance, and that the overuse and misuse in both animals and humans is a key factor in the acquisition of resistance genes. The discovery of multidrug resistance strains in both communities and hospitals is a wakeup call to take on this problem that raises the morbidity and mortality rates for strains that were sensible to specific antibiotics in the past, such as Staphylococcus aureus to penicillin. There is a need for creation of new drugs and new therapeutic approaches. In this investigation, MIC testing was done to test the efficiency of two conventional antibiotics; Ampicillin and Tetracycline, and one antimicrobial peptide; Polymyxin B. The purpose of this was to find out whether or not antimicrobial peptides are an efficient and effective alternative to conventional antibiotics. Through these experiments, it was concluded that Polymyxin B was a great alternative to both Ampicillin and Tetracycline in the treatment of Escherichia coli. 3,9µg/ml of Polymyxin B was needed in the experiment to inhibit bacterial growth, compared to 62,5µg/ml of Tetracycline. However, due to its natural properties, Polymyxin B was not effective against Staphylococcus aureus. In response to the results observed when tested on E. coli, it can be concluded that antimicrobial peptides can and should be used as an alternative to conventional antibiotics, for the treatment of bacteria, such as E. coli. This can potentially help solve the problem of antibiotic resistant strains of bacteria. With more antimicrobial drugs on the market, physicians will have more options when treating patients for bacterial infections, and mortality due to infection will decrease as a result.

UddannelserBasis - International Naturvidenskabelig Bacheloruddannelse, (Bachelor uddannelse) Basis
SprogEngelsk
Udgivelsesdato14 jan. 2013
VejledereBiljana Mojsoska

Emneord

  • Antimicrobial
  • peptides
  • resistance