Anti-coagulation drugs and mechanism

Hossein Khademi & Natalia Andersen

Studenteropgave: Semesterprojekt

Abstrakt

In this project there has been an attempt to study the coagulation cascade and anti-coagulants. The problem formulation of this project is ‘‘Can the shallowness of the dose-effect curve indicate Argatroban (a DTI) to be more effective in prevention of thrombosis than Unfractioned Heparin, where aPTT method is used on plasma samples in vitro?’’. The method used in the experiments was activated Partial Thromboplastin Time (aPTT). The normal assay consisted of addition of platelin, plasma and Ca2+ in equal volumes of 100 μl in a glass tube inside 37 °C water bath along with 10 μl of saline. The glass tube was tilted after the addition of Ca2+ and the time taken for the clot to appear in the tube after the addition of Ca2+ was measured and named as reference clotting time. The method was also applied by changing other variables such as the dilution Ca2+ in saline and dilution of plasma in saline while keeping the other variables constant. Moreover, the method was also applied to Heparin, where Heparin was added in dilutions of saline instead of the 10 μl saline. The same method as Heparin was applied on Argatroban in order to have the dose-effect curves of both anticoagulants for comparison. In the results related to the problem formulation, the dose-effect curve of Argatroban in vitro was steeper than the dose-effect curve of Heparin in vitro. Therefore the dose-effect curve of Heparin in vitro is actually, unlike what originally expected, shallower and more predictable than the doseffect curve of Argatroban in vitro. On the other hand, the literature showed a better dose-effect curve of Argatroban than Heparin. Therefore in conclusion the results of our experiments cannot be a good indicator for studying and comparing the effects of Argatroban to Heparin.

UddannelserBasis - International Naturvidenskabelig Bacheloruddannelse, (Bachelor uddannelse) Basis
SprogEngelsk
Udgivelsesdato23 jun. 2010

Emneord

  • Anticoagulants
  • aPTT
  • Direct Thrombin Inhibitors
  • Coagulation
  • Argatroban
  • Heparin