An investigation on the potential bioaccumulation effects in the freshwater sediment dwelling worm Tubifex tubifex exposed to pyrene and the potential vector effect of microplastic

Patrick Jack Vognstoft, Rasmus Tronier Hansen, Johann Nathan Blom Schnügger & Martine Pedersen Lynderup

Studenteropgave: Bachelorprojekt


The problematic amount of chemicals in our water systems and the missing amount of microplastic in our oceans give rise to the question of what the fate of these is. This report address one aspect of this issue by investigating bioaccumulation of a known contaminant (pyrene) to the sediment dwelling oligochaete Tubifex tubifex in a controlled experiment. The focus point of this project is the fate of both the polycyclic aromatic hydrocarbons (PAH) chemicals, specifically pyrene, as well as microplastic, specifically polyethylene. The experiment was run at 20℃ ± 4℃ for five days and consisted of 24 glass measuring cylinders with five T. tubifex in each. We made 12 measuring cylinders containing ~0.18 kg sediment, 0 mg, 1 mg, 5 mg, and 10 mg pyrene per kg sediment and 12 measuring cylinders containing ~0.18 kg sediment, 0 mg, 1 mg, 5 mg, and 10 mg pyrene per kg sediment, and ~0.117 g polyethylene microbeads. The results show bioaccumulation factors of 1.201, 1.476, and 0.006 for 1 mg, 5 mg, and 10 mg pyrene, respectively and 2.281, 3.714, and 0.001 for 1 mg, 5 mg, and 10 mg pyrene sorbed to microplastic. The conclusion is that we observed a Trojan horse effect in low concentrations of pyrene, as microplastic increased the bioaccumulation of pyrene in T. tubifex.

UddannelserBasis - Naturvidenskabelig Bacheloruddannelse, (Bachelor uddannelse) BachelorBasis - International Naturvidenskabelig Bacheloruddannelse, (Bachelor uddannelse) Bachelor
Udgivelsesdato27 maj 2018
Antal sider33
VejledereKristian Syberg


  • Tubifex tubifex
  • Pyrene
  • Microplastic
  • Polyethylene
  • Bioaccumulation
  • BAF
  • Vector effect
  • Oligochaete
  • Freshwater
  • Sediment
  • Freshwater sediment
  • PAH
  • Polycyclic aromatic hydrocarbons
  • Environment