An Assessment of the Delayed Effects of Exposure to CuO Nanoparticles and CuCl2 Spiked Sediment on Mortality and Burrowing Behaviour of the Freshwater Annelid Tubifex tubifex

Anne Holm, Lucas Absalon, Michael Jørgensen & Nayia Charalambous

Studenteropgave: Semesterprojekt


Copper nanoparticles are being used more and more frequently in our everyday life; this leads to the question of what the effects on the environment and organisms living in the contaminated ecosystems are. This report seeks to help answering this question, by researching if the sedimentassociated copper (two forms, CuO NPs or Cu-ions) exposure has a delayed effects on the mortality and burrowing behaviour of the annelid Tubifex tubifex after an exposure period of four days. This is done by exposing T.tubifex to sediment spiked with either CuO NPs or Cu-ions (sequestered as CuCl2 + 2H2O) for 4 days, followed by a transfer to clean (non-spiked) sediment for an additional 4 days. Delayed effects are determined as a change in mortality and burrowing behaviour of the worms. It was found that in this setup the Cu, especially in the NP form, had a delayed effect on T. tubifex. This was shown by both an increase in mortality and a lower ability to burrow into the sediment, when transferred to the clean sediment. This indicates that attention should be paid to not only the short term effects, but also the delayed effects before being able to predict the outcome of NP products.

UddannelserBasis - Naturvidenskabelig Bacheloruddannelse, (Bachelor uddannelse) BasisBasis - International Naturvidenskabelig Bacheloruddannelse, (Bachelor uddannelse) Basis
Udgivelsesdato22 jun. 2015


  • tubifex
  • nanoparticles
  • Ecosystem
  • copper
  • ecotoxicity
  • sediment