An approach to adhere body cavity cells of Tardigrada through the model organism Drosophila melanogaster

Louise Brinck, Kristian Meier, Jonas B. Aagren, Jens T. Pedersen, Rikki F. Frederiksen & Allan A. Kristensen

Studenteropgave: Semesterprojekt

Abstrakt

The aim of this review is to find the most efficient way of adhering body cavity cells from Tardigrada to a surface without killing it. This is of interest because these cells are believed to be involved in anhydrobiose. By conducting an atomic force microscope (AFM) scan of these cells it is expected that more knowledge will be obtained. Little is known about the body cavity cells, so in order to fulfil our aim we have chosen to look at another invertebrate that is well described in the literature, being Drosophila melanogaster, which is closely related to Tardigrada. In order to compare these organisms we assume that fat body cells from D. melanogaster have the same type of adhesion properties as the body cavity cells from Tardigrada. In this review integrins, RGD, fibronectin and the components of the basal lamina; laminin, collagen, nidogen, and the proteoglycans perlecan and papilin are considered. Of these we recommend RGD as a coating medium before testing other more difficult and expensive coating mediums. If no adhesion is observed with RGD another approach would be to coat with laminin, either the whole protein or the different binding sequences independently.

UddannelserMolekylærbiologi, (Bachelor/kandidatuddannelse) Bachelor el. kandidat
SprogEngelsk
Udgivelsesdato1 jun. 2006
VejledereOle Vang

Emneord

  • Tardigrada
  • Integrin
  • Laminin
  • Body cavity cells
  • Collagen
  • Cell adhesion
  • perlecan
  • Basal lamina
  • tiggrin
  • papilin
  • Drosophila melanogaster
  • RGD