Two thymidine kinases and one multisubstrate deoxyribonucleoside kinase salvage DNA precursors in Arabidopsis thaliana

Anders Ranegaard Anders Ranegaard Clausen, Lenart Girandon, Ashfaq Ali, Wolfgang Knecht, Elzbieta Rozpedowska, Michael Paolo Sandrini, Erik Andreasson, Birgitte Munch-Petersen, Jure Piskur

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    Resumé

    Deoxyribonucleotides are the building blocks of DNA and can be synthesized via de novo and salvage pathways. Deoxyribonucleoside kinases (EC 2.7.1.145) salvage deoxyribonucleosides by transfer of a phosphate group to the 5' of a deoxyribonucleoside. This salvage pathway is well characterized in mammals, but in contrast, little is known about how plants salvage deoxyribonucleosides. We show that during salvage, deoxyribonucleosides can be phosphorylated by extracts of Arabidopsis thaliana into corresponding monophosphate compounds with an unexpected preference for purines over pyrimidines. Deoxyribonucleoside kinase activities were present in all tissues during all growth stages. In the A. thaliana genome, we identified two types of genes that could encode enzymes which are involved in the salvage of deoxyribonucleosides. Thymidine kinase activity was encoded by two thymidine kinase 1 (EC 2.7.1.21)-like genes (AtTK1a and AtTK1b). Deoxyadenosine, deoxyguanosine and deoxycytidine kinase activities were encoded by a single AtdNK gene. T-DNA insertion lines of AtTK1a and AtTK1b mutant genes had normal growth, although AtTK1a AtTK1b double mutants died at an early stage, which indicates that AtTK1a and AtTK1b catalyze redundant reactions. The results obtained in the present study suggest a crucial role for the salvage of thymidine during early plant development.
    OriginalsprogEngelsk
    TidsskriftF E B S Journal
    Vol/bind279
    Udgave nummer20
    Sider (fra-til)3889-3897
    Antal sider9
    ISSN1742-464X
    DOI
    StatusUdgivet - jan. 2012

    Emneord

    • Arabidopsis
    • nucleoside nucleotide metabolism
    • deoxyribonucleoside kinase
    • thymidine kinase

    Citer dette

    Anders Ranegaard Clausen, A. R., Girandon, L., Ali, A., Knecht, W., Rozpedowska, E., Sandrini, M. P., ... Piskur, J. (2012). Two thymidine kinases and one multisubstrate deoxyribonucleoside kinase salvage DNA precursors in Arabidopsis thaliana. F E B S Journal, 279(20), 3889-3897. https://doi.org/0.1111/j.1742-4658.2012.08747.x
    Anders Ranegaard Clausen, Anders Ranegaard ; Girandon, Lenart ; Ali, Ashfaq ; Knecht, Wolfgang ; Rozpedowska, Elzbieta ; Sandrini, Michael Paolo ; Andreasson, Erik ; Munch-Petersen, Birgitte ; Piskur, Jure. / Two thymidine kinases and one multisubstrate deoxyribonucleoside kinase salvage DNA precursors in Arabidopsis thaliana. I: F E B S Journal. 2012 ; Bind 279, Nr. 20. s. 3889-3897.
    @article{c5542210b7314414aae81be0cf5fcd54,
    title = "Two thymidine kinases and one multisubstrate deoxyribonucleoside kinase salvage DNA precursors in Arabidopsis thaliana",
    abstract = "Deoxyribonucleotides are the building blocks of DNA and can be synthesized via de novo and salvage pathways. Deoxyribonucleoside kinases (EC 2.7.1.145) salvage deoxyribonucleosides by transfer of a phosphate group to the 5' of a deoxyribonucleoside. This salvage pathway is well characterized in mammals, but in contrast, little is known about how plants salvage deoxyribonucleosides. We show that during salvage, deoxyribonucleosides can be phosphorylated by extracts of Arabidopsis thaliana into corresponding monophosphate compounds with an unexpected preference for purines over pyrimidines. Deoxyribonucleoside kinase activities were present in all tissues during all growth stages. In the A. thaliana genome, we identified two types of genes that could encode enzymes which are involved in the salvage of deoxyribonucleosides. Thymidine kinase activity was encoded by two thymidine kinase 1 (EC 2.7.1.21)-like genes (AtTK1a and AtTK1b). Deoxyadenosine, deoxyguanosine and deoxycytidine kinase activities were encoded by a single AtdNK gene. T-DNA insertion lines of AtTK1a and AtTK1b mutant genes had normal growth, although AtTK1a AtTK1b double mutants died at an early stage, which indicates that AtTK1a and AtTK1b catalyze redundant reactions. The results obtained in the present study suggest a crucial role for the salvage of thymidine during early plant development.",
    keywords = "Arabidopsis , nucleoside nucleotide metabolism , deoxyribonucleoside kinase, thymidine kinase",
    author = "{Anders Ranegaard Clausen}, {Anders Ranegaard} and Lenart Girandon and Ashfaq Ali and Wolfgang Knecht and Elzbieta Rozpedowska and Sandrini, {Michael Paolo} and Erik Andreasson and Birgitte Munch-Petersen and Jure Piskur",
    year = "2012",
    month = "1",
    doi = "0.1111/j.1742-4658.2012.08747.x",
    language = "English",
    volume = "279",
    pages = "3889--3897",
    journal = "F E B S Journal",
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    Anders Ranegaard Clausen, AR, Girandon, L, Ali, A, Knecht, W, Rozpedowska, E, Sandrini, MP, Andreasson, E, Munch-Petersen, B & Piskur, J 2012, 'Two thymidine kinases and one multisubstrate deoxyribonucleoside kinase salvage DNA precursors in Arabidopsis thaliana', F E B S Journal, bind 279, nr. 20, s. 3889-3897. https://doi.org/0.1111/j.1742-4658.2012.08747.x

    Two thymidine kinases and one multisubstrate deoxyribonucleoside kinase salvage DNA precursors in Arabidopsis thaliana. / Anders Ranegaard Clausen, Anders Ranegaard ; Girandon, Lenart; Ali, Ashfaq; Knecht, Wolfgang; Rozpedowska, Elzbieta; Sandrini, Michael Paolo; Andreasson, Erik; Munch-Petersen, Birgitte; Piskur, Jure.

    I: F E B S Journal, Bind 279, Nr. 20, 01.2012, s. 3889-3897.

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    TY - JOUR

    T1 - Two thymidine kinases and one multisubstrate deoxyribonucleoside kinase salvage DNA precursors in Arabidopsis thaliana

    AU - Anders Ranegaard Clausen, Anders Ranegaard

    AU - Girandon, Lenart

    AU - Ali, Ashfaq

    AU - Knecht, Wolfgang

    AU - Rozpedowska, Elzbieta

    AU - Sandrini, Michael Paolo

    AU - Andreasson, Erik

    AU - Munch-Petersen, Birgitte

    AU - Piskur, Jure

    PY - 2012/1

    Y1 - 2012/1

    N2 - Deoxyribonucleotides are the building blocks of DNA and can be synthesized via de novo and salvage pathways. Deoxyribonucleoside kinases (EC 2.7.1.145) salvage deoxyribonucleosides by transfer of a phosphate group to the 5' of a deoxyribonucleoside. This salvage pathway is well characterized in mammals, but in contrast, little is known about how plants salvage deoxyribonucleosides. We show that during salvage, deoxyribonucleosides can be phosphorylated by extracts of Arabidopsis thaliana into corresponding monophosphate compounds with an unexpected preference for purines over pyrimidines. Deoxyribonucleoside kinase activities were present in all tissues during all growth stages. In the A. thaliana genome, we identified two types of genes that could encode enzymes which are involved in the salvage of deoxyribonucleosides. Thymidine kinase activity was encoded by two thymidine kinase 1 (EC 2.7.1.21)-like genes (AtTK1a and AtTK1b). Deoxyadenosine, deoxyguanosine and deoxycytidine kinase activities were encoded by a single AtdNK gene. T-DNA insertion lines of AtTK1a and AtTK1b mutant genes had normal growth, although AtTK1a AtTK1b double mutants died at an early stage, which indicates that AtTK1a and AtTK1b catalyze redundant reactions. The results obtained in the present study suggest a crucial role for the salvage of thymidine during early plant development.

    AB - Deoxyribonucleotides are the building blocks of DNA and can be synthesized via de novo and salvage pathways. Deoxyribonucleoside kinases (EC 2.7.1.145) salvage deoxyribonucleosides by transfer of a phosphate group to the 5' of a deoxyribonucleoside. This salvage pathway is well characterized in mammals, but in contrast, little is known about how plants salvage deoxyribonucleosides. We show that during salvage, deoxyribonucleosides can be phosphorylated by extracts of Arabidopsis thaliana into corresponding monophosphate compounds with an unexpected preference for purines over pyrimidines. Deoxyribonucleoside kinase activities were present in all tissues during all growth stages. In the A. thaliana genome, we identified two types of genes that could encode enzymes which are involved in the salvage of deoxyribonucleosides. Thymidine kinase activity was encoded by two thymidine kinase 1 (EC 2.7.1.21)-like genes (AtTK1a and AtTK1b). Deoxyadenosine, deoxyguanosine and deoxycytidine kinase activities were encoded by a single AtdNK gene. T-DNA insertion lines of AtTK1a and AtTK1b mutant genes had normal growth, although AtTK1a AtTK1b double mutants died at an early stage, which indicates that AtTK1a and AtTK1b catalyze redundant reactions. The results obtained in the present study suggest a crucial role for the salvage of thymidine during early plant development.

    KW - Arabidopsis

    KW - nucleoside nucleotide metabolism

    KW - deoxyribonucleoside kinase

    KW - thymidine kinase

    U2 - 0.1111/j.1742-4658.2012.08747.x

    DO - 0.1111/j.1742-4658.2012.08747.x

    M3 - Journal article

    VL - 279

    SP - 3889

    EP - 3897

    JO - F E B S Journal

    JF - F E B S Journal

    SN - 1742-464X

    IS - 20

    ER -

    Anders Ranegaard Clausen AR, Girandon L, Ali A, Knecht W, Rozpedowska E, Sandrini MP et al. Two thymidine kinases and one multisubstrate deoxyribonucleoside kinase salvage DNA precursors in Arabidopsis thaliana. F E B S Journal. 2012 jan;279(20):3889-3897. https://doi.org/0.1111/j.1742-4658.2012.08747.x