Trophic transfer of CuO NPs and dissolved Cu from sediment to worms to fish - a proof-of-concept study

Tobias Lammel, Amalie Thit Jensen, Catherine Mouneyrac, Anders Baun, Joachim Sturve, Henriette Selck

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Man-made nanoparticles (NPs) released into the aquatic environment are likely to accumulate in sediment. Uptake of NPs by benthic invertebrates that serve as food for higher trophic level organisms such as fish may result in their transfer along the food chain. We tested this hypothesis in a proof-of-concept approach consisting of two studies examining the transfer of copper (II) oxide NPs (CuO NPs) (<50 nm) and dissolved copper (CuCl2). Study 1 focussed on transfer from sediment to worms (Tubifex tubifex), Study 2 on transfer from worms (T. tubifex) to fish (Gasterosteus aculeatus, three-spined stickleback). A key methodological achievement of Study 2 was the development of worm homogenate-based food packages, which allowed controlling CuO NP and CuCl2 dosing of fish. In T. tubifex, no significant differences in Cu accumulation were detected following 7 days of exposure to sediment-associated CuO NPs or CuCl2 (100 µg Cu/g dw sediment). Yet, metallothionein-like protein levels were significantly elevated in CuO NP-exposed worms compared to the control, while they were not in CuCl2-exposed worms. G. aculeatus fed CuO NP and CuCl2-spiked food packages (2 µg Cu·g-1 fish ww·day-1; 7 days) showed increased Cu concentrations (~13 and ~31 µg Cu/g dw compared to control, respectively) and metallothionein (mta) mRNA expression in intestinal tissue. In CuO NP-exposed fish Cu and mta mRNA levels were noticeably lower than in CuCl2-exposed and inversely correlated with the amount of Cu in their faeces suggesting that a large fraction of NPs was egested. Altogether, the results suggest that sediment-associated CuO NPs can enter the aquatic food web, but that transfer of CuO NPs from T. tubifex to stickleback may be limited compared to dissolved Cu. Furthermore, there were no indications for induction of oxidative stress in fish intestine and liver suggesting that effects from environmentally relevant Cu/CuO NP exposure via the diet may be minimal.
OriginalsprogEngelsk
TidsskriftEnvironmental Science: Nano
Vol/bind2019
Udgave nummer4
ISSN2051-8153
DOI
StatusUdgivet - 2019

Citer dette

@article{42bf085ff22e4607b10cb5e04c64b16f,
title = "Trophic transfer of CuO NPs and dissolved Cu from sediment to worms to fish - a proof-of-concept study",
abstract = "Man-made nanoparticles (NPs) released into the aquatic environment are likely to accumulate in sediment. Uptake of NPs by benthic invertebrates that serve as food for higher trophic level organisms such as fish may result in their transfer along the food chain. We tested this hypothesis in a proof-of-concept approach consisting of two studies examining the transfer of copper (II) oxide NPs (CuO NPs) (<50 nm) and dissolved copper (CuCl2). Study 1 focussed on transfer from sediment to worms (Tubifex tubifex), Study 2 on transfer from worms (T. tubifex) to fish (Gasterosteus aculeatus, three-spined stickleback). A key methodological achievement of Study 2 was the development of worm homogenate-based food packages, which allowed controlling CuO NP and CuCl2 dosing of fish. In T. tubifex, no significant differences in Cu accumulation were detected following 7 days of exposure to sediment-associated CuO NPs or CuCl2 (100 µg Cu/g dw sediment). Yet, metallothionein-like protein levels were significantly elevated in CuO NP-exposed worms compared to the control, while they were not in CuCl2-exposed worms. G. aculeatus fed CuO NP and CuCl2-spiked food packages (2 µg Cu·g-1 fish ww·day-1; 7 days) showed increased Cu concentrations (~13 and ~31 µg Cu/g dw compared to control, respectively) and metallothionein (mta) mRNA expression in intestinal tissue. In CuO NP-exposed fish Cu and mta mRNA levels were noticeably lower than in CuCl2-exposed and inversely correlated with the amount of Cu in their faeces suggesting that a large fraction of NPs was egested. Altogether, the results suggest that sediment-associated CuO NPs can enter the aquatic food web, but that transfer of CuO NPs from T. tubifex to stickleback may be limited compared to dissolved Cu. Furthermore, there were no indications for induction of oxidative stress in fish intestine and liver suggesting that effects from environmentally relevant Cu/CuO NP exposure via the diet may be minimal.",
author = "Tobias Lammel and Jensen, {Amalie Thit} and Catherine Mouneyrac and Anders Baun and Joachim Sturve and Henriette Selck",
year = "2019",
doi = "10.1039/C9EN00093C",
language = "English",
volume = "2019",
journal = "Environmental Science: Nano",
issn = "2051-8153",
publisher = "RSC Publications",
number = "4",

}

Trophic transfer of CuO NPs and dissolved Cu from sediment to worms to fish - a proof-of-concept study. / Lammel, Tobias; Jensen, Amalie Thit; Mouneyrac, Catherine; Baun, Anders; Sturve, Joachim; Selck, Henriette.

I: Environmental Science: Nano, Bind 2019, Nr. 4, 2019.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Trophic transfer of CuO NPs and dissolved Cu from sediment to worms to fish - a proof-of-concept study

AU - Lammel, Tobias

AU - Jensen, Amalie Thit

AU - Mouneyrac, Catherine

AU - Baun, Anders

AU - Sturve, Joachim

AU - Selck, Henriette

PY - 2019

Y1 - 2019

N2 - Man-made nanoparticles (NPs) released into the aquatic environment are likely to accumulate in sediment. Uptake of NPs by benthic invertebrates that serve as food for higher trophic level organisms such as fish may result in their transfer along the food chain. We tested this hypothesis in a proof-of-concept approach consisting of two studies examining the transfer of copper (II) oxide NPs (CuO NPs) (<50 nm) and dissolved copper (CuCl2). Study 1 focussed on transfer from sediment to worms (Tubifex tubifex), Study 2 on transfer from worms (T. tubifex) to fish (Gasterosteus aculeatus, three-spined stickleback). A key methodological achievement of Study 2 was the development of worm homogenate-based food packages, which allowed controlling CuO NP and CuCl2 dosing of fish. In T. tubifex, no significant differences in Cu accumulation were detected following 7 days of exposure to sediment-associated CuO NPs or CuCl2 (100 µg Cu/g dw sediment). Yet, metallothionein-like protein levels were significantly elevated in CuO NP-exposed worms compared to the control, while they were not in CuCl2-exposed worms. G. aculeatus fed CuO NP and CuCl2-spiked food packages (2 µg Cu·g-1 fish ww·day-1; 7 days) showed increased Cu concentrations (~13 and ~31 µg Cu/g dw compared to control, respectively) and metallothionein (mta) mRNA expression in intestinal tissue. In CuO NP-exposed fish Cu and mta mRNA levels were noticeably lower than in CuCl2-exposed and inversely correlated with the amount of Cu in their faeces suggesting that a large fraction of NPs was egested. Altogether, the results suggest that sediment-associated CuO NPs can enter the aquatic food web, but that transfer of CuO NPs from T. tubifex to stickleback may be limited compared to dissolved Cu. Furthermore, there were no indications for induction of oxidative stress in fish intestine and liver suggesting that effects from environmentally relevant Cu/CuO NP exposure via the diet may be minimal.

AB - Man-made nanoparticles (NPs) released into the aquatic environment are likely to accumulate in sediment. Uptake of NPs by benthic invertebrates that serve as food for higher trophic level organisms such as fish may result in their transfer along the food chain. We tested this hypothesis in a proof-of-concept approach consisting of two studies examining the transfer of copper (II) oxide NPs (CuO NPs) (<50 nm) and dissolved copper (CuCl2). Study 1 focussed on transfer from sediment to worms (Tubifex tubifex), Study 2 on transfer from worms (T. tubifex) to fish (Gasterosteus aculeatus, three-spined stickleback). A key methodological achievement of Study 2 was the development of worm homogenate-based food packages, which allowed controlling CuO NP and CuCl2 dosing of fish. In T. tubifex, no significant differences in Cu accumulation were detected following 7 days of exposure to sediment-associated CuO NPs or CuCl2 (100 µg Cu/g dw sediment). Yet, metallothionein-like protein levels were significantly elevated in CuO NP-exposed worms compared to the control, while they were not in CuCl2-exposed worms. G. aculeatus fed CuO NP and CuCl2-spiked food packages (2 µg Cu·g-1 fish ww·day-1; 7 days) showed increased Cu concentrations (~13 and ~31 µg Cu/g dw compared to control, respectively) and metallothionein (mta) mRNA expression in intestinal tissue. In CuO NP-exposed fish Cu and mta mRNA levels were noticeably lower than in CuCl2-exposed and inversely correlated with the amount of Cu in their faeces suggesting that a large fraction of NPs was egested. Altogether, the results suggest that sediment-associated CuO NPs can enter the aquatic food web, but that transfer of CuO NPs from T. tubifex to stickleback may be limited compared to dissolved Cu. Furthermore, there were no indications for induction of oxidative stress in fish intestine and liver suggesting that effects from environmentally relevant Cu/CuO NP exposure via the diet may be minimal.

U2 - 10.1039/C9EN00093C

DO - 10.1039/C9EN00093C

M3 - Journal article

VL - 2019

JO - Environmental Science: Nano

JF - Environmental Science: Nano

SN - 2051-8153

IS - 4

ER -