Toxic effects and bioaccumulation of nano-, micron- and ionic-Ag in the polychaete, Nereis diversicolor

Yi cong, Gary Thomas Banta, Henriette Selck, Deborah Berhanu, Eugenia Valsami-Jones, Valery E. Forbes

Publikation: KonferencebidragPosterForskning

Resumé

There is increasing concern about the toxicities and potential risks, both still poorly understood, of silver nanoparticles for the aquatic environment after their eventual release. In this study, the toxicities of nano (< 100 nm)-, micron (2-3.5 µm)- and ionic (AgNO3)-Ag on the sediment-dwelling polychaete, Nereis diversicolor, were compared after 10 d of sediment exposure, using growth, DNA damage (comet assay) and bioaccumulation as endpoints. The nominal concentrations used in all exposure scenarios were 0, 1, 5, 10, 25, 50 µg Ag/g dry weight (dw) sediment. Our results show that Ag is able to cause DNA damage in Nereis coelomocytes and that this effect is both concentration- and Ag form-related. There were significantly greater genotoxity (higher tail moment and tail DNA intensities) at 25 and 50 µg/g dw in nano- and micron-Ag treated groups and at 50 µg/g dw in ionic-Ag treated group compared to the controls (0 µg/g dw). Nano-Ag has the greatest genotoxic effect of the three tested Ag forms and ionic-Ag is the least genotoxic, indicating different mechanisms are possibly involved to cause DNA damage by the different Ag forms. N. diversicolor did accumulate sediment-associated Ag from all three forms. Significant increases in Ag body burdens in worms treated with ionic-Ag (5, 10, 25, 50 µg/g dw), nano- and micron-Ag (25, 50 µg/g dw) were observed compared to the controls. Ag body burdens at the highest exposure concentration were 9.86±4.94, 8.56±6.63 and 6.92±5.86 µg/g dw for ionic-, nano- and micron-Ag treated worms, respectively. However, there are no form- or size-related differences in bioaccumulation of Ag. No measurable change in wet weight for any of the worm groups during the 10 d experiment was observed, indicating that this measure of growth is not a useful endpoint for detecting effects of such short duration exposures for this species.
OriginalsprogEngelsk
Publikationsdato1 mar. 2011
StatusUdgivet - 1 mar. 2011
BegivenhedSETAC young scientist conference: Environmental challenges in a changing world - RWTH Aachen University, Aachen, Tyskland
Varighed: 28 feb. 20112 mar. 2011

Konference

KonferenceSETAC young scientist conference
LokationRWTH Aachen University
LandTyskland
ByAachen
Periode28/02/201102/03/2011

Citer dette

cong, Y., Banta, G. T., Selck, H., Berhanu, D., Valsami-Jones, E., & Forbes, V. E. (2011). Toxic effects and bioaccumulation of nano-, micron- and ionic-Ag in the polychaete, Nereis diversicolor. Poster session præsenteret på SETAC young scientist conference, Aachen, Tyskland.
cong, Yi ; Banta, Gary Thomas ; Selck, Henriette ; Berhanu, Deborah ; Valsami-Jones, Eugenia ; Forbes, Valery E. / Toxic effects and bioaccumulation of nano-, micron- and ionic-Ag in the polychaete, Nereis diversicolor. Poster session præsenteret på SETAC young scientist conference, Aachen, Tyskland.
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title = "Toxic effects and bioaccumulation of nano-, micron- and ionic-Ag in the polychaete, Nereis diversicolor",
abstract = "There is increasing concern about the toxicities and potential risks, both still poorly understood, of silver nanoparticles for the aquatic environment after their eventual release. In this study, the toxicities of nano (< 100 nm)-, micron (2-3.5 µm)- and ionic (AgNO3)-Ag on the sediment-dwelling polychaete, Nereis diversicolor, were compared after 10 d of sediment exposure, using growth, DNA damage (comet assay) and bioaccumulation as endpoints. The nominal concentrations used in all exposure scenarios were 0, 1, 5, 10, 25, 50 µg Ag/g dry weight (dw) sediment. Our results show that Ag is able to cause DNA damage in Nereis coelomocytes and that this effect is both concentration- and Ag form-related. There were significantly greater genotoxity (higher tail moment and tail DNA intensities) at 25 and 50 µg/g dw in nano- and micron-Ag treated groups and at 50 µg/g dw in ionic-Ag treated group compared to the controls (0 µg/g dw). Nano-Ag has the greatest genotoxic effect of the three tested Ag forms and ionic-Ag is the least genotoxic, indicating different mechanisms are possibly involved to cause DNA damage by the different Ag forms. N. diversicolor did accumulate sediment-associated Ag from all three forms. Significant increases in Ag body burdens in worms treated with ionic-Ag (5, 10, 25, 50 µg/g dw), nano- and micron-Ag (25, 50 µg/g dw) were observed compared to the controls. Ag body burdens at the highest exposure concentration were 9.86±4.94, 8.56±6.63 and 6.92±5.86 µg/g dw for ionic-, nano- and micron-Ag treated worms, respectively. However, there are no form- or size-related differences in bioaccumulation of Ag. No measurable change in wet weight for any of the worm groups during the 10 d experiment was observed, indicating that this measure of growth is not a useful endpoint for detecting effects of such short duration exposures for this species.",
author = "Yi cong and Banta, {Gary Thomas} and Henriette Selck and Deborah Berhanu and Eugenia Valsami-Jones and Forbes, {Valery E.}",
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cong, Y, Banta, GT, Selck, H, Berhanu, D, Valsami-Jones, E & Forbes, VE 2011, 'Toxic effects and bioaccumulation of nano-, micron- and ionic-Ag in the polychaete, Nereis diversicolor' SETAC young scientist conference, Aachen, Tyskland, 28/02/2011 - 02/03/2011, .

Toxic effects and bioaccumulation of nano-, micron- and ionic-Ag in the polychaete, Nereis diversicolor. / cong, Yi; Banta, Gary Thomas; Selck, Henriette; Berhanu, Deborah ; Valsami-Jones, Eugenia ; Forbes, Valery E.

2011. Poster session præsenteret på SETAC young scientist conference, Aachen, Tyskland.

Publikation: KonferencebidragPosterForskning

TY - CONF

T1 - Toxic effects and bioaccumulation of nano-, micron- and ionic-Ag in the polychaete, Nereis diversicolor

AU - cong, Yi

AU - Banta, Gary Thomas

AU - Selck, Henriette

AU - Berhanu, Deborah

AU - Valsami-Jones, Eugenia

AU - Forbes, Valery E.

PY - 2011/3/1

Y1 - 2011/3/1

N2 - There is increasing concern about the toxicities and potential risks, both still poorly understood, of silver nanoparticles for the aquatic environment after their eventual release. In this study, the toxicities of nano (< 100 nm)-, micron (2-3.5 µm)- and ionic (AgNO3)-Ag on the sediment-dwelling polychaete, Nereis diversicolor, were compared after 10 d of sediment exposure, using growth, DNA damage (comet assay) and bioaccumulation as endpoints. The nominal concentrations used in all exposure scenarios were 0, 1, 5, 10, 25, 50 µg Ag/g dry weight (dw) sediment. Our results show that Ag is able to cause DNA damage in Nereis coelomocytes and that this effect is both concentration- and Ag form-related. There were significantly greater genotoxity (higher tail moment and tail DNA intensities) at 25 and 50 µg/g dw in nano- and micron-Ag treated groups and at 50 µg/g dw in ionic-Ag treated group compared to the controls (0 µg/g dw). Nano-Ag has the greatest genotoxic effect of the three tested Ag forms and ionic-Ag is the least genotoxic, indicating different mechanisms are possibly involved to cause DNA damage by the different Ag forms. N. diversicolor did accumulate sediment-associated Ag from all three forms. Significant increases in Ag body burdens in worms treated with ionic-Ag (5, 10, 25, 50 µg/g dw), nano- and micron-Ag (25, 50 µg/g dw) were observed compared to the controls. Ag body burdens at the highest exposure concentration were 9.86±4.94, 8.56±6.63 and 6.92±5.86 µg/g dw for ionic-, nano- and micron-Ag treated worms, respectively. However, there are no form- or size-related differences in bioaccumulation of Ag. No measurable change in wet weight for any of the worm groups during the 10 d experiment was observed, indicating that this measure of growth is not a useful endpoint for detecting effects of such short duration exposures for this species.

AB - There is increasing concern about the toxicities and potential risks, both still poorly understood, of silver nanoparticles for the aquatic environment after their eventual release. In this study, the toxicities of nano (< 100 nm)-, micron (2-3.5 µm)- and ionic (AgNO3)-Ag on the sediment-dwelling polychaete, Nereis diversicolor, were compared after 10 d of sediment exposure, using growth, DNA damage (comet assay) and bioaccumulation as endpoints. The nominal concentrations used in all exposure scenarios were 0, 1, 5, 10, 25, 50 µg Ag/g dry weight (dw) sediment. Our results show that Ag is able to cause DNA damage in Nereis coelomocytes and that this effect is both concentration- and Ag form-related. There were significantly greater genotoxity (higher tail moment and tail DNA intensities) at 25 and 50 µg/g dw in nano- and micron-Ag treated groups and at 50 µg/g dw in ionic-Ag treated group compared to the controls (0 µg/g dw). Nano-Ag has the greatest genotoxic effect of the three tested Ag forms and ionic-Ag is the least genotoxic, indicating different mechanisms are possibly involved to cause DNA damage by the different Ag forms. N. diversicolor did accumulate sediment-associated Ag from all three forms. Significant increases in Ag body burdens in worms treated with ionic-Ag (5, 10, 25, 50 µg/g dw), nano- and micron-Ag (25, 50 µg/g dw) were observed compared to the controls. Ag body burdens at the highest exposure concentration were 9.86±4.94, 8.56±6.63 and 6.92±5.86 µg/g dw for ionic-, nano- and micron-Ag treated worms, respectively. However, there are no form- or size-related differences in bioaccumulation of Ag. No measurable change in wet weight for any of the worm groups during the 10 d experiment was observed, indicating that this measure of growth is not a useful endpoint for detecting effects of such short duration exposures for this species.

M3 - Poster

ER -

cong Y, Banta GT, Selck H, Berhanu D, Valsami-Jones E, Forbes VE. Toxic effects and bioaccumulation of nano-, micron- and ionic-Ag in the polychaete, Nereis diversicolor. 2011. Poster session præsenteret på SETAC young scientist conference, Aachen, Tyskland.