Time-Dependent Subcellular Distribution and Effects of Carbon Nanotubes in Lungs of Mice

Carsten Købler, Anne Saber, Nicklas R. Jacobsen, Håkan Wallin, Ulla Vogel, Kristian Mølhave, Carole Yauk, Sabina Halappanavar, Klaus Qvortrup, Sarah Søs Poulsen

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    Resumé

    Background and Methods
    Pulmonary deposited carbon nanotubes (CNTs) are cleared very slowly from the lung, but there is limited information on how CNTs interact with the lung tissue over time. To address this, three different multiwalled CNTs were intratracheally instilled into female C57BL/6 mice: one short (850 nm) and tangled, and two longer (4 μm and 5.7 μm) and thicker. We assessed the cellular interaction with these CNTs using transmission electron microscopy (TEM) 1, 3 and 28 days after instillation.
    Results
    TEM analysis revealed that the three CNTs followed the same overall progression pattern over time. Initially, CNTs were taken up either by a diffusion mechanism or via endocytosis. Then CNTs were agglomerated in vesicles in macrophages. Lastly, at 28 days post-exposure, evidence suggesting CNT escape from vesicle enclosures were found. The longer and thicker CNTs more often perturbed and escaped vesicular enclosures in macrophages compared to the smaller CNTs. Bronchoalveolar lavage (BAL) showed that the CNT exposure induced both an eosinophil influx and also eosinophilic crystalline pneumonia.
    Conclusion
    Two very different types of multiwalled CNTs had very similar pattern of cellular interactions in lung tissue, with the longer and thicker CNTs resulting in more severe effects in terms of eosinophil influx and incidence of eosinophilic crystalline pneumonia (ECP)
    OriginalsprogEngelsk
    TidsskriftP L o S One
    ISSN1932-6203
    DOI
    StatusUdgivet - 23 jan. 2015

    Citer dette

    Købler, Carsten ; Saber, Anne ; Jacobsen, Nicklas R. ; Wallin, Håkan ; Vogel, Ulla ; Mølhave, Kristian ; Yauk, Carole ; Halappanavar, Sabina ; Qvortrup, Klaus ; Poulsen, Sarah Søs. / Time-Dependent Subcellular Distribution and Effects of Carbon Nanotubes in Lungs of Mice. I: P L o S One. 2015.
    @article{16a4ccd0e0004d89ad1a1ba8d7992b0c,
    title = "Time-Dependent Subcellular Distribution and Effects of Carbon Nanotubes in Lungs of Mice",
    abstract = "Background and MethodsPulmonary deposited carbon nanotubes (CNTs) are cleared very slowly from the lung, but there is limited information on how CNTs interact with the lung tissue over time. To address this, three different multiwalled CNTs were intratracheally instilled into female C57BL/6 mice: one short (850 nm) and tangled, and two longer (4 μm and 5.7 μm) and thicker. We assessed the cellular interaction with these CNTs using transmission electron microscopy (TEM) 1, 3 and 28 days after instillation.ResultsTEM analysis revealed that the three CNTs followed the same overall progression pattern over time. Initially, CNTs were taken up either by a diffusion mechanism or via endocytosis. Then CNTs were agglomerated in vesicles in macrophages. Lastly, at 28 days post-exposure, evidence suggesting CNT escape from vesicle enclosures were found. The longer and thicker CNTs more often perturbed and escaped vesicular enclosures in macrophages compared to the smaller CNTs. Bronchoalveolar lavage (BAL) showed that the CNT exposure induced both an eosinophil influx and also eosinophilic crystalline pneumonia.ConclusionTwo very different types of multiwalled CNTs had very similar pattern of cellular interactions in lung tissue, with the longer and thicker CNTs resulting in more severe effects in terms of eosinophil influx and incidence of eosinophilic crystalline pneumonia (ECP)",
    author = "Carsten K{\o}bler and Anne Saber and Jacobsen, {Nicklas R.} and H{\aa}kan Wallin and Ulla Vogel and Kristian M{\o}lhave and Carole Yauk and Sabina Halappanavar and Klaus Qvortrup and Poulsen, {Sarah S{\o}s}",
    year = "2015",
    month = "1",
    day = "23",
    doi = "10.1371/journal.pone.0116481",
    language = "English",
    journal = "P L o S One",
    issn = "1932-6203",
    publisher = "Public Library of Science",

    }

    Købler, C, Saber, A, Jacobsen, NR, Wallin, H, Vogel, U, Mølhave, K, Yauk, C, Halappanavar, S, Qvortrup, K & Poulsen, SS 2015, 'Time-Dependent Subcellular Distribution and Effects of Carbon Nanotubes in Lungs of Mice', P L o S One. https://doi.org/10.1371/journal.pone.0116481

    Time-Dependent Subcellular Distribution and Effects of Carbon Nanotubes in Lungs of Mice. / Købler, Carsten; Saber, Anne ; Jacobsen, Nicklas R.; Wallin, Håkan; Vogel, Ulla; Mølhave, Kristian; Yauk, Carole; Halappanavar, Sabina; Qvortrup, Klaus; Poulsen, Sarah Søs.

    I: P L o S One, 23.01.2015.

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    TY - JOUR

    T1 - Time-Dependent Subcellular Distribution and Effects of Carbon Nanotubes in Lungs of Mice

    AU - Købler, Carsten

    AU - Saber, Anne

    AU - Jacobsen, Nicklas R.

    AU - Wallin, Håkan

    AU - Vogel, Ulla

    AU - Mølhave, Kristian

    AU - Yauk, Carole

    AU - Halappanavar, Sabina

    AU - Qvortrup, Klaus

    AU - Poulsen, Sarah Søs

    PY - 2015/1/23

    Y1 - 2015/1/23

    N2 - Background and MethodsPulmonary deposited carbon nanotubes (CNTs) are cleared very slowly from the lung, but there is limited information on how CNTs interact with the lung tissue over time. To address this, three different multiwalled CNTs were intratracheally instilled into female C57BL/6 mice: one short (850 nm) and tangled, and two longer (4 μm and 5.7 μm) and thicker. We assessed the cellular interaction with these CNTs using transmission electron microscopy (TEM) 1, 3 and 28 days after instillation.ResultsTEM analysis revealed that the three CNTs followed the same overall progression pattern over time. Initially, CNTs were taken up either by a diffusion mechanism or via endocytosis. Then CNTs were agglomerated in vesicles in macrophages. Lastly, at 28 days post-exposure, evidence suggesting CNT escape from vesicle enclosures were found. The longer and thicker CNTs more often perturbed and escaped vesicular enclosures in macrophages compared to the smaller CNTs. Bronchoalveolar lavage (BAL) showed that the CNT exposure induced both an eosinophil influx and also eosinophilic crystalline pneumonia.ConclusionTwo very different types of multiwalled CNTs had very similar pattern of cellular interactions in lung tissue, with the longer and thicker CNTs resulting in more severe effects in terms of eosinophil influx and incidence of eosinophilic crystalline pneumonia (ECP)

    AB - Background and MethodsPulmonary deposited carbon nanotubes (CNTs) are cleared very slowly from the lung, but there is limited information on how CNTs interact with the lung tissue over time. To address this, three different multiwalled CNTs were intratracheally instilled into female C57BL/6 mice: one short (850 nm) and tangled, and two longer (4 μm and 5.7 μm) and thicker. We assessed the cellular interaction with these CNTs using transmission electron microscopy (TEM) 1, 3 and 28 days after instillation.ResultsTEM analysis revealed that the three CNTs followed the same overall progression pattern over time. Initially, CNTs were taken up either by a diffusion mechanism or via endocytosis. Then CNTs were agglomerated in vesicles in macrophages. Lastly, at 28 days post-exposure, evidence suggesting CNT escape from vesicle enclosures were found. The longer and thicker CNTs more often perturbed and escaped vesicular enclosures in macrophages compared to the smaller CNTs. Bronchoalveolar lavage (BAL) showed that the CNT exposure induced both an eosinophil influx and also eosinophilic crystalline pneumonia.ConclusionTwo very different types of multiwalled CNTs had very similar pattern of cellular interactions in lung tissue, with the longer and thicker CNTs resulting in more severe effects in terms of eosinophil influx and incidence of eosinophilic crystalline pneumonia (ECP)

    U2 - 10.1371/journal.pone.0116481

    DO - 10.1371/journal.pone.0116481

    M3 - Journal article

    JO - P L o S One

    JF - P L o S One

    SN - 1932-6203

    ER -