The two chromosomes of Vibrio cholerae are initiated at different time points in the cell cycle

Tue Rasmussen, Rasmus Bugge Jensen, Ole Skovgaard

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    Resumé

    The bacterium Vibrio cholerae, the cause of the diarrhoeal disease cholera, has its genome divided between two chromosomes, a feature uncommon for bacteria. The two chromosomes are of different sizes and different initiator molecules control their replication independently. Using novel methods for analysing flow cytometry data and marker frequency analysis, we show that the small chromosome II is replicated late in the C period of the cell cycle, where most of chromosome I has been replicated. Owing to the delay in initiation of chromosome II, the two chromosomes terminate replication at approximately the same time and the average number of replication origins per cell is higher for chromosome I than for chromosome II. Analysis of cell-cycle parameters shows that chromosome replication and segregation is exceptionally fast in V. cholerae. The divided genome and delayed replication of chromosome II may reduce the metabolic burden and complexity of chromosome replication by postponing DNA synthesis to the last part of the cell cycle and reducing the need for overlapping replication cycles during rapid proliferation
    OriginalsprogEngelsk
    TidsskriftEMBO Journal
    Vol/bind26
    Udgave nummer13
    Sider (fra-til)3124-3131
    Antal sider8
    ISSN0261-4189
    DOI
    StatusUdgivet - 2007

    Citer dette

    Rasmussen, Tue ; Jensen, Rasmus Bugge ; Skovgaard, Ole. / The two chromosomes of Vibrio cholerae are initiated at different time points in the cell cycle. I: EMBO Journal. 2007 ; Bind 26, Nr. 13. s. 3124-3131.
    @article{4c4a5060a3e111dcaf2d000ea68e967b,
    title = "The two chromosomes of Vibrio cholerae are initiated at different time points in the cell cycle",
    abstract = "The bacterium Vibrio cholerae, the cause of the diarrhoeal disease cholera, has its genome divided between two chromosomes, a feature uncommon for bacteria. The two chromosomes are of different sizes and different initiator molecules control their replication independently. Using novel methods for analysing flow cytometry data and marker frequency analysis, we show that the small chromosome II is replicated late in the C period of the cell cycle, where most of chromosome I has been replicated. Owing to the delay in initiation of chromosome II, the two chromosomes terminate replication at approximately the same time and the average number of replication origins per cell is higher for chromosome I than for chromosome II. Analysis of cell-cycle parameters shows that chromosome replication and segregation is exceptionally fast in V. cholerae. The divided genome and delayed replication of chromosome II may reduce the metabolic burden and complexity of chromosome replication by postponing DNA synthesis to the last part of the cell cycle and reducing the need for overlapping replication cycles during rapid proliferation",
    keywords = "C period, divided genomes, flow cytometry, initiation of DNA replication, replication time",
    author = "Tue Rasmussen and Jensen, {Rasmus Bugge} and Ole Skovgaard",
    year = "2007",
    doi = "10.1038/sj.emboj.7601747",
    language = "English",
    volume = "26",
    pages = "3124--3131",
    journal = "E M B O Journal",
    issn = "0261-4189",
    publisher = "Wiley-Blackwell Publishing Ltd.",
    number = "13",

    }

    The two chromosomes of Vibrio cholerae are initiated at different time points in the cell cycle. / Rasmussen, Tue; Jensen, Rasmus Bugge; Skovgaard, Ole.

    I: EMBO Journal, Bind 26, Nr. 13, 2007, s. 3124-3131.

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    TY - JOUR

    T1 - The two chromosomes of Vibrio cholerae are initiated at different time points in the cell cycle

    AU - Rasmussen, Tue

    AU - Jensen, Rasmus Bugge

    AU - Skovgaard, Ole

    PY - 2007

    Y1 - 2007

    N2 - The bacterium Vibrio cholerae, the cause of the diarrhoeal disease cholera, has its genome divided between two chromosomes, a feature uncommon for bacteria. The two chromosomes are of different sizes and different initiator molecules control their replication independently. Using novel methods for analysing flow cytometry data and marker frequency analysis, we show that the small chromosome II is replicated late in the C period of the cell cycle, where most of chromosome I has been replicated. Owing to the delay in initiation of chromosome II, the two chromosomes terminate replication at approximately the same time and the average number of replication origins per cell is higher for chromosome I than for chromosome II. Analysis of cell-cycle parameters shows that chromosome replication and segregation is exceptionally fast in V. cholerae. The divided genome and delayed replication of chromosome II may reduce the metabolic burden and complexity of chromosome replication by postponing DNA synthesis to the last part of the cell cycle and reducing the need for overlapping replication cycles during rapid proliferation

    AB - The bacterium Vibrio cholerae, the cause of the diarrhoeal disease cholera, has its genome divided between two chromosomes, a feature uncommon for bacteria. The two chromosomes are of different sizes and different initiator molecules control their replication independently. Using novel methods for analysing flow cytometry data and marker frequency analysis, we show that the small chromosome II is replicated late in the C period of the cell cycle, where most of chromosome I has been replicated. Owing to the delay in initiation of chromosome II, the two chromosomes terminate replication at approximately the same time and the average number of replication origins per cell is higher for chromosome I than for chromosome II. Analysis of cell-cycle parameters shows that chromosome replication and segregation is exceptionally fast in V. cholerae. The divided genome and delayed replication of chromosome II may reduce the metabolic burden and complexity of chromosome replication by postponing DNA synthesis to the last part of the cell cycle and reducing the need for overlapping replication cycles during rapid proliferation

    KW - C period

    KW - divided genomes

    KW - flow cytometry

    KW - initiation of DNA replication

    KW - replication time

    U2 - 10.1038/sj.emboj.7601747

    DO - 10.1038/sj.emboj.7601747

    M3 - Journal article

    VL - 26

    SP - 3124

    EP - 3131

    JO - E M B O Journal

    JF - E M B O Journal

    SN - 0261-4189

    IS - 13

    ER -