The Sabatier principle as a tool for discovery and engineering of industrial enzymes

Jeppe Kari, Kay S. Schaller, Gustavo A Molina, Kim Borch, Peter Westh*

*Corresponding author

Publikation: Bidrag til tidsskriftReviewpeer review

Abstract

The recent breakthrough in all-atom, protein structure prediction opens new avenues for a range of computational approaches in enzyme design. These new approaches could become instrumental for the development of technical biocatalysts, and hence our transition toward more sustainable industries. Here, we discuss one approach, which is well-known within inorganic catalysis, but essentially unexploited in biotechnology. Specifically, we review examples of linear free-energy relationships (LFERs) for enzyme reactions and discuss how LFERs and the associated Sabatier Principle may be implemented in algorithms that estimate kinetic parameters and enzyme performance based on model structures.
OriginalsprogEngelsk
Artikelnummer102843
TidsskriftCurrent Opinion in Biotechnology
Vol/bind78
ISSN0958-1669
DOI
StatusUdgivet - dec. 2022

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