The role of protonation in protein fibrillation

Martin Jeppesen, Peter Westh, Daniel Otzen

Publikation: Bidrag til tidsskriftTidsskriftartikelpeer review

Abstract

Many proteins fibrillate at low pH despite a high population of charged side chains. Therefore exchange of protons between the fibrillating peptide and its surroundings may play an important role in fibrillation. Here, we use isothermal titration calorimetry to measure exchange of protons between buffer and the peptide hormone glucagon during fibrillation. Glucagon absorbs or releases protons to an extent which allows it to attain a net charge of zero in the fibrillar state, both at acidic and basic pH. Similar results are obtained for lysozyme. This suggests that side chain pKa values change dramatically in the fibrillar state.
OriginalsprogEngelsk
TidsskriftFEBS Letters
Vol/bind584
Udgave nummer4
Sider (fra-til)780-784
ISSN0014-5793
DOI
StatusUdgivet - 2010

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