The Role of Decorated SDS Micelles in Sub-CMC Protein Denaturation and Association

Kell Andersen, Cristiano Luis Pinto De Oliveira, K.L. Larsen, Flemming Poulsen, Thomas Hønger Callisen, Peter Westh, Jan Skov Pedersen, Daniel Otzen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

We have combined spectroscopy, chromatography, calorimetry, and small-angle X-ray scattering (SAXS) to provide a comprehensive structural and stoichiometric description of the sodium dodecyl sulfate (SDS)-induced denaturation of the 86-residue α-helical bovine acyl-coenzyme-A-binding protein (ACBP). Denaturation is a multistep process. Initial weak binding of 1-3 SDS molecules per protein molecule below 1.3 mM does not perturb the tertiary structure. Subsequent binding of  13 SDS molecules per ACBP molecule leads to the formation of SDS aggregates on the protein and changes in both tertiary and secondary structures. SAXS data show that, at this stage, a decorated micelle links two ACBP molecules together, leaving about half of the polypeptide chain as a disordered region protruding into the solvent. Further titration with SDS leads to the additional uptake of 26 SDS molecules, which, according to SAXS, forms a larger decorated micelle bound to a single ACBP molecule. At the critical micelle concentration, we conclude from reduced mobility and increased fluorescence anisotropy that each ACBP molecule becomes associated with more than one micelle. At this point, 56-60 SDS molecules are bound per ACBP molecule. Our data provide key structural insights into decorated micelle complexes with proteins, revealing a remarkable diversity in the different conformations they can stabilize. The data highlight that a minimum decorated micelle size, which may be a key driving force for intermolecular protein association, exists. This may also provide a structural basis for the known ability of submicellar surfactant concentrations to induce protein aggregation and fibrillation.
OriginalsprogEngelsk
TidsskriftJournal of Molecular Biology
Vol/bind391
Udgave nummer1
Sider (fra-til)207-226
ISSN0022-2836
DOI
StatusUdgivet - 2009

Citer dette

Andersen, K., Oliveira, C. L. P. D., Larsen, K. L., Poulsen, F., Callisen, T. H., Westh, P., ... Otzen, D. (2009). The Role of Decorated SDS Micelles in Sub-CMC Protein Denaturation and Association. Journal of Molecular Biology, 391(1), 207-226. https://doi.org/10.1016/j.jmb.2009.06.019
Andersen, Kell ; Oliveira, Cristiano Luis Pinto De ; Larsen, K.L. ; Poulsen, Flemming ; Callisen, Thomas Hønger ; Westh, Peter ; Pedersen, Jan Skov ; Otzen, Daniel. / The Role of Decorated SDS Micelles in Sub-CMC Protein Denaturation and Association. I: Journal of Molecular Biology. 2009 ; Bind 391, Nr. 1. s. 207-226.
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abstract = "We have combined spectroscopy, chromatography, calorimetry, and small-angle X-ray scattering (SAXS) to provide a comprehensive structural and stoichiometric description of the sodium dodecyl sulfate (SDS)-induced denaturation of the 86-residue α-helical bovine acyl-coenzyme-A-binding protein (ACBP). Denaturation is a multistep process. Initial weak binding of 1-3 SDS molecules per protein molecule below 1.3 mM does not perturb the tertiary structure. Subsequent binding of  13 SDS molecules per ACBP molecule leads to the formation of SDS aggregates on the protein and changes in both tertiary and secondary structures. SAXS data show that, at this stage, a decorated micelle links two ACBP molecules together, leaving about half of the polypeptide chain as a disordered region protruding into the solvent. Further titration with SDS leads to the additional uptake of 26 SDS molecules, which, according to SAXS, forms a larger decorated micelle bound to a single ACBP molecule. At the critical micelle concentration, we conclude from reduced mobility and increased fluorescence anisotropy that each ACBP molecule becomes associated with more than one micelle. At this point, 56-60 SDS molecules are bound per ACBP molecule. Our data provide key structural insights into decorated micelle complexes with proteins, revealing a remarkable diversity in the different conformations they can stabilize. The data highlight that a minimum decorated micelle size, which may be a key driving force for intermolecular protein association, exists. This may also provide a structural basis for the known ability of submicellar surfactant concentrations to induce protein aggregation and fibrillation.",
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Andersen, K, Oliveira, CLPD, Larsen, KL, Poulsen, F, Callisen, TH, Westh, P, Pedersen, JS & Otzen, D 2009, 'The Role of Decorated SDS Micelles in Sub-CMC Protein Denaturation and Association', Journal of Molecular Biology, bind 391, nr. 1, s. 207-226. https://doi.org/10.1016/j.jmb.2009.06.019

The Role of Decorated SDS Micelles in Sub-CMC Protein Denaturation and Association. / Andersen, Kell; Oliveira, Cristiano Luis Pinto De; Larsen, K.L.; Poulsen, Flemming; Callisen, Thomas Hønger; Westh, Peter; Pedersen, Jan Skov; Otzen, Daniel.

I: Journal of Molecular Biology, Bind 391, Nr. 1, 2009, s. 207-226.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - The Role of Decorated SDS Micelles in Sub-CMC Protein Denaturation and Association

AU - Andersen, Kell

AU - Oliveira, Cristiano Luis Pinto De

AU - Larsen, K.L.

AU - Poulsen, Flemming

AU - Callisen, Thomas Hønger

AU - Westh, Peter

AU - Pedersen, Jan Skov

AU - Otzen, Daniel

PY - 2009

Y1 - 2009

N2 - We have combined spectroscopy, chromatography, calorimetry, and small-angle X-ray scattering (SAXS) to provide a comprehensive structural and stoichiometric description of the sodium dodecyl sulfate (SDS)-induced denaturation of the 86-residue α-helical bovine acyl-coenzyme-A-binding protein (ACBP). Denaturation is a multistep process. Initial weak binding of 1-3 SDS molecules per protein molecule below 1.3 mM does not perturb the tertiary structure. Subsequent binding of  13 SDS molecules per ACBP molecule leads to the formation of SDS aggregates on the protein and changes in both tertiary and secondary structures. SAXS data show that, at this stage, a decorated micelle links two ACBP molecules together, leaving about half of the polypeptide chain as a disordered region protruding into the solvent. Further titration with SDS leads to the additional uptake of 26 SDS molecules, which, according to SAXS, forms a larger decorated micelle bound to a single ACBP molecule. At the critical micelle concentration, we conclude from reduced mobility and increased fluorescence anisotropy that each ACBP molecule becomes associated with more than one micelle. At this point, 56-60 SDS molecules are bound per ACBP molecule. Our data provide key structural insights into decorated micelle complexes with proteins, revealing a remarkable diversity in the different conformations they can stabilize. The data highlight that a minimum decorated micelle size, which may be a key driving force for intermolecular protein association, exists. This may also provide a structural basis for the known ability of submicellar surfactant concentrations to induce protein aggregation and fibrillation.

AB - We have combined spectroscopy, chromatography, calorimetry, and small-angle X-ray scattering (SAXS) to provide a comprehensive structural and stoichiometric description of the sodium dodecyl sulfate (SDS)-induced denaturation of the 86-residue α-helical bovine acyl-coenzyme-A-binding protein (ACBP). Denaturation is a multistep process. Initial weak binding of 1-3 SDS molecules per protein molecule below 1.3 mM does not perturb the tertiary structure. Subsequent binding of  13 SDS molecules per ACBP molecule leads to the formation of SDS aggregates on the protein and changes in both tertiary and secondary structures. SAXS data show that, at this stage, a decorated micelle links two ACBP molecules together, leaving about half of the polypeptide chain as a disordered region protruding into the solvent. Further titration with SDS leads to the additional uptake of 26 SDS molecules, which, according to SAXS, forms a larger decorated micelle bound to a single ACBP molecule. At the critical micelle concentration, we conclude from reduced mobility and increased fluorescence anisotropy that each ACBP molecule becomes associated with more than one micelle. At this point, 56-60 SDS molecules are bound per ACBP molecule. Our data provide key structural insights into decorated micelle complexes with proteins, revealing a remarkable diversity in the different conformations they can stabilize. The data highlight that a minimum decorated micelle size, which may be a key driving force for intermolecular protein association, exists. This may also provide a structural basis for the known ability of submicellar surfactant concentrations to induce protein aggregation and fibrillation.

KW - ACBP

KW - surfactant

KW - isothermal titration calorimetry

KW - small-angle X-ray scattering

KW - dimerization

U2 - 10.1016/j.jmb.2009.06.019

DO - 10.1016/j.jmb.2009.06.019

M3 - Journal article

VL - 391

SP - 207

EP - 226

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 1

ER -