The Mobilome: Metagenomic Analysis of Circular Plasmids, Viruses, and Other Extrachromosomal Elements

Patrick Denis Browne, Witold Kot, Tue Sparholt Jørgensen, Lars Hestbjerg Hansen*

*Corresponding author

Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiForskningpeer review


Isolation, sequencing, and analysis of circular genetic elements bring new insights to mobile genetic elements related to microbial ecology. One method used to study circular plasmids, viruses, and other elements is called the mobilome method. The mobilome method presented here is an unamplified mobilome approach allowing fast isolation of circular DNA elements from a variety of samples followed by directly building unamplified Illumina-compatible sequencing libraries using enzymatic tagging and fragmentation. Several methods for bioinformatic analysis of mobilome data are also suggested.

TitelMethods in Molecular Biology
Antal sider12
ForlagHumana Press
ISBN (Trykt)978-1-4939-9876-0
ISBN (Elektronisk)978-1-4939-9876-0
StatusUdgivet - 2020
NavnMethods in Molecular Biology

Bibliografisk note

Funding Information:
The authors gratefully acknowledge support from the Villum Foundation (Block Stipend to LHH & PDB and AMPHICOP project no. 8960 to TSJ), Lundbeck Foundation (grant no. R44-A4384 LHH and TSJ), and Danish Council for Independent Research (grant no. 4093-00198 awarded to WK).

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2020.

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