Isolation, sequencing, and analysis of circular genetic elements bring new insights to mobile genetic elements related to microbial ecology. One method used to study circular plasmids, viruses, and other elements is called the mobilome method. The mobilome method presented here is an unamplified mobilome approach allowing fast isolation of circular DNA elements from a variety of samples followed by directly building unamplified Illumina-compatible sequencing libraries using enzymatic tagging and fragmentation. Several methods for bioinformatic analysis of mobilome data are also suggested.
|Titel||Methods in Molecular Biology|
|Status||Udgivet - 2020|
|Navn||Methods in Molecular Biology|
Bibliografisk noteFunding Information:
The authors gratefully acknowledge support from the Villum Foundation (Block Stipend to LHH & PDB and AMPHICOP project no. 8960 to TSJ), Lundbeck Foundation (grant no. R44-A4384 LHH and TSJ), and Danish Council for Independent Research (grant no. 4093-00198 awarded to WK).
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