The Halloween genes code for cytochrome P450 enzymes mediating synthesis of the insect molting hormone

Kim Rewitz, Robert Rybczynski, James T. Warren, Lawrence I. Gilbert

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskning

    Resumé

    Udgivelsesdato: December
    OriginalsprogEngelsk
    TidsskriftBiochemical Society Transactions
    Vol/bind34
    Udgave nummer6
    Sider (fra-til)1256-1260
    Antal sider5
    ISSN0300-5127
    StatusUdgivet - 2006

    Citer dette

    Rewitz, K., Rybczynski, R., Warren, J. T., & Gilbert, L. I. (2006). The Halloween genes code for cytochrome P450 enzymes mediating synthesis of the insect molting hormone. Biochemical Society Transactions, 34(6), 1256-1260.
    Rewitz, Kim ; Rybczynski, Robert ; Warren, James T. ; Gilbert, Lawrence I. / The Halloween genes code for cytochrome P450 enzymes mediating synthesis of the insect molting hormone. I: Biochemical Society Transactions. 2006 ; Bind 34, Nr. 6. s. 1256-1260.
    @article{60b570a0d2ec11db9c7c000ea68e967b,
    title = "The Halloween genes code for cytochrome P450 enzymes mediating synthesis of the insect molting hormone",
    abstract = "The developmental events occurring during molting and metamorphosis of insects are controlled by precisely timed changes in levels of ecdysteroids, the molting hormones. The final four sequential hydroxylations of steroid precursors into the active ecdysteroid of insects, 20-hydroxyecdysone (20E), are mediated by four cytochrome P450 enzymes, encoded by genes in the Halloween family. Orthologs of the Drosophila Halloween genes phantom (phm: CYP306A1), disembodied (dib: CYP302A1), shadow (sad: CYP315A1) and shade (shd: CYP314A1) were obtained from the endocrinological model insect, the tobacco hornworm Manduca sexta. Expression of these genes was studied and compared with changes in ecdysteroid titer that controls transition from the larval to pupal stage. Phm, dib and sad (mediating the final hydroxylations in the biosynthesis of ecdysone; E) were selectively expressed in the prothoracic gland, the primary source of E during larval and pupal development. Changes in their expression correlate with the hemolymph ecdysteroid titer during the fifth (final) larval instar. Shd, the 20-hydroxylase, which converts E into the more active 20E, is expressed in tissues peripheral to the prothoracic glands during the fifth instar. Transcript levels of shd in the fat body and midgut closely parallel the enzyme activity measured in vitro. The data indicate that these Halloween genes are transcriptionally regulated to support the high biosynthetic activity that produces the cyclic ecdysteroid pulses triggering molting.",
    keywords = "Halloween, P450, Molting, Development, Insect, Ecdysteroids, ecdysone",
    author = "Kim Rewitz and Robert Rybczynski and Warren, {James T.} and Gilbert, {Lawrence I.}",
    year = "2006",
    language = "English",
    volume = "34",
    pages = "1256--1260",
    journal = "Biochemical Society Transactions",
    issn = "0300-5127",
    publisher = "Portland Press Ltd.",
    number = "6",

    }

    Rewitz, K, Rybczynski, R, Warren, JT & Gilbert, LI 2006, 'The Halloween genes code for cytochrome P450 enzymes mediating synthesis of the insect molting hormone', Biochemical Society Transactions, bind 34, nr. 6, s. 1256-1260.

    The Halloween genes code for cytochrome P450 enzymes mediating synthesis of the insect molting hormone. / Rewitz, Kim; Rybczynski, Robert; Warren, James T.; Gilbert, Lawrence I.

    I: Biochemical Society Transactions, Bind 34, Nr. 6, 2006, s. 1256-1260.

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskning

    TY - JOUR

    T1 - The Halloween genes code for cytochrome P450 enzymes mediating synthesis of the insect molting hormone

    AU - Rewitz, Kim

    AU - Rybczynski, Robert

    AU - Warren, James T.

    AU - Gilbert, Lawrence I.

    PY - 2006

    Y1 - 2006

    N2 - The developmental events occurring during molting and metamorphosis of insects are controlled by precisely timed changes in levels of ecdysteroids, the molting hormones. The final four sequential hydroxylations of steroid precursors into the active ecdysteroid of insects, 20-hydroxyecdysone (20E), are mediated by four cytochrome P450 enzymes, encoded by genes in the Halloween family. Orthologs of the Drosophila Halloween genes phantom (phm: CYP306A1), disembodied (dib: CYP302A1), shadow (sad: CYP315A1) and shade (shd: CYP314A1) were obtained from the endocrinological model insect, the tobacco hornworm Manduca sexta. Expression of these genes was studied and compared with changes in ecdysteroid titer that controls transition from the larval to pupal stage. Phm, dib and sad (mediating the final hydroxylations in the biosynthesis of ecdysone; E) were selectively expressed in the prothoracic gland, the primary source of E during larval and pupal development. Changes in their expression correlate with the hemolymph ecdysteroid titer during the fifth (final) larval instar. Shd, the 20-hydroxylase, which converts E into the more active 20E, is expressed in tissues peripheral to the prothoracic glands during the fifth instar. Transcript levels of shd in the fat body and midgut closely parallel the enzyme activity measured in vitro. The data indicate that these Halloween genes are transcriptionally regulated to support the high biosynthetic activity that produces the cyclic ecdysteroid pulses triggering molting.

    AB - The developmental events occurring during molting and metamorphosis of insects are controlled by precisely timed changes in levels of ecdysteroids, the molting hormones. The final four sequential hydroxylations of steroid precursors into the active ecdysteroid of insects, 20-hydroxyecdysone (20E), are mediated by four cytochrome P450 enzymes, encoded by genes in the Halloween family. Orthologs of the Drosophila Halloween genes phantom (phm: CYP306A1), disembodied (dib: CYP302A1), shadow (sad: CYP315A1) and shade (shd: CYP314A1) were obtained from the endocrinological model insect, the tobacco hornworm Manduca sexta. Expression of these genes was studied and compared with changes in ecdysteroid titer that controls transition from the larval to pupal stage. Phm, dib and sad (mediating the final hydroxylations in the biosynthesis of ecdysone; E) were selectively expressed in the prothoracic gland, the primary source of E during larval and pupal development. Changes in their expression correlate with the hemolymph ecdysteroid titer during the fifth (final) larval instar. Shd, the 20-hydroxylase, which converts E into the more active 20E, is expressed in tissues peripheral to the prothoracic glands during the fifth instar. Transcript levels of shd in the fat body and midgut closely parallel the enzyme activity measured in vitro. The data indicate that these Halloween genes are transcriptionally regulated to support the high biosynthetic activity that produces the cyclic ecdysteroid pulses triggering molting.

    KW - Halloween

    KW - P450

    KW - Molting

    KW - Development

    KW - Insect

    KW - Ecdysteroids

    KW - ecdysone

    M3 - Journal article

    VL - 34

    SP - 1256

    EP - 1260

    JO - Biochemical Society Transactions

    JF - Biochemical Society Transactions

    SN - 0300-5127

    IS - 6

    ER -