The Applicability of a Short-term Test for Detection of Modifying Effects of Dietary Factors in Rodent Colon Carcinogenesis

The present studies were initiated to develop a short-term
rodent model to assess the influence of different dietary
components on the development of colon cancer.
Diets with different dietary components, i.e. dietary fibre, fat,
sucrose, and starches were tested in male rats initiated with
DMH-2HCl or AOM for their modulating effect on the
development of aberrant crypt foci (ACF). Furthermore the
heterocyclic amines IQ and PhIP were introduced in the assay
as inducers of ACF in mice and rats and their role in colon
carcinogenesis in mice was investigated.
ACF were found to be induced in rodent colon by the colon
carcinogens DMH-2HC1, AOM, IQ, and PhIP and it was
shown that the incidence of the induced ACF could be
modulated by dietary components such as sucrose, dietary
fibre, and starch.