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A dual column photobioreactor (PBR) (2 × 47 L) with mixed CO2/air bubbling was tested for cultivation of the microalga Rhodomonas salina as food for live feed copepods. In the continuous growth phase, the cell density was relatively stable at 2.40 ± 0.13 × 106 cells/ml at an average dilution rate of 0.46 ± 0.02 per day throughout the 30‐day experiment. The produced algae had a high content of both total fatty acids (TFA) and free amino acids (FAA). Especially, the harvested algae contained a high proportion of poly‐unsaturated fatty acids that made up 80% of the TFA and of essential amino acids (35% of all FAA), implicating desirable components as feed for copepods. The current PBR was sufficient to feed a culture of the calanoid copepod Acartia tonsa at a density of 2,500 adult/L in ca. 500 L culture with a daily yield of approximately 17 × 106 eggs. To be able to sustain the integrated copepods production, the suggested volume of the algae cultures should be ca. 20% of the copepod culture volume.
Thuy, M. V. T., Jepsen, P. M., Jørgensen, N. O. G., Hansen, B. W., & Nielsen, S. L. (2018). Testing the yield of a pilot-scale bubble column photobioreactor for cultivation of the microalga Rhodomonas salina as feed for intensive calanoid copepod cultures. Aquaculture Research, 50(1), 63-71. https://doi.org/10.1111/are.13868