Techniques and applications of NMR to membrane proteins

N.Chr. Nielsen*, A. Malmendal, T. Vosegaard

*Corresponding author

Publikation: Bidrag til tidsskriftReviewForskningpeer review

Abstract

The fact that membrane proteins are notoriously difficult to analyse using standard protocols for atomic-resolution structure determination methods have motivated adaptation of these techniques to membrane protein studies as well as development of new technologies. With this motivation, liquid-state nuclear magnetic resonance (NMR) has recently been used with success for studies of peptides and membrane proteins in detergent micelles, and solid-state NMR has undergone a tremendous evolution towards characterization of membrane proteins in native membrane and oriented phospholipid bilayers. In this mini-review, we describe some of the technological challenges behind these efforts and provide examples on their use in membrane biology.
OriginalsprogEngelsk
TidsskriftMolecular Membrane Biology
Vol/bind21
Udgave nummer3
Sider (fra-til)129-141
ISSN0968-7688
DOI
StatusUdgivet - 2004
Udgivet eksterntJa

Bibliografisk note

This article has been found as a ’Free Version’ from the Publisher on June 23 2020. When access to the article closes, please notify rucforsk@ruc.dk

Emneord

  • Alamethicin
  • Bacteriorhodopsin
  • Ca -ATPase 2+
  • Membrane proteins
  • NMR spectroscopy
  • Structure determination

Citer dette