Abstract
The fact that membrane proteins are notoriously difficult to analyse using standard protocols for atomic-resolution structure determination methods have motivated adaptation of these techniques to membrane protein studies as well as development of new technologies. With this motivation, liquid-state nuclear magnetic resonance (NMR) has recently been used with success for studies of peptides and membrane proteins in detergent micelles, and solid-state NMR has undergone a tremendous evolution towards characterization of membrane proteins in native membrane and oriented phospholipid bilayers. In this mini-review, we describe some of the technological challenges behind these efforts and provide examples on their use in membrane biology.
Originalsprog | Engelsk |
---|---|
Tidsskrift | Molecular Membrane Biology |
Vol/bind | 21 |
Udgave nummer | 3 |
Sider (fra-til) | 129-141 |
ISSN | 0968-7688 |
DOI | |
Status | Udgivet - 2004 |
Udgivet eksternt | Ja |
Bibliografisk note
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- Alamethicin
- Bacteriorhodopsin
- Ca -ATPase 2+
- Membrane proteins
- NMR spectroscopy
- Structure determination