Stimuli-responsive polymersomes of poly [2-(dimethylamino) ethyl methacrylate]-b-polystyrene

Valdomiro V. de Souza; Gustavo P.B. Carretero; Phelipe A.M. Vitale; Íris Todeschini; Paloma O. Kotani; Greice K.V. Saraiva; Cristiane R. Guzzo; Hernan Chaimovich; Fabio H. Florenzano; Iolanda M. Cuccovia

doi:10.1007/s00289-020-03533-5

Stimuli-responsive polymersomes of poly [2-(dimethylamino) ethyl methacrylate]-b-polystyrene

Valdomiro V. de Souza, Gustavo P.B. Carretero, Phelipe A.M. Vitale, Íris Todeschini, Paloma O. Kotani, Greice K.V. Saraiva, Cristiane R. Guzzo, Hernan Chaimovich, Fabio H. Florenzano^*, Iolanda M. Cuccovia

^*Corresponding author

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Abstract

Amphiphilic diblock copolymers may assemble in aqueous solutions to form vesicles delimited by a polymeric double layer, also known as polymersomes, considered a more robust option to liposomes. Diblock copolymers may respond to pH, temperature, and other conditions. Because of such properties, polymersomes are currently being studied as drug delivery systems or as nanoreactors. pH-responsive polymersomes are potentially crucial because unusual pH gradients are present in cells under several physiological and pathological conditions. We synthesized two diblock copolymers of poly [2-(dimethylamino) ethyl methacrylate]-block-polystyrene (PDMAEMA-b-PS) via RAFT. We both developed new materials and better-understood polymersomes' properties with pH and temperature-responsive groups with these polymers. GPC, 1H-NMR, and FTIR characterized copolymers. The ionization equilibrium of the PDMAEMA amino groups on the polymersomes was analyzed by potentiometric titration and Zeta potential measurements. The hydrodynamic radius of the polymersomes in different pH and temperatures was analyzed by DLS. Entrapment of an electron paramagnetic resonance probe indicated the presence of a hydrophilic inner core. Negative staining transmission electron microscopy showed spherical aggregates and confirmed the diameter around 80 nm. These polymersomes with dual stimulus–response (i.e., pH and temperature) may be a platform for gene delivery and nanoreactors.

Originalsprog	Engelsk
Tidsskrift	Polymer Bulletin
Vol/bind	79
Udgave nummer	2
Sider (fra-til)	785-805
Antal sider	21
ISSN	0170-0839
DOI	https://doi.org/10.1007/s00289-020-03533-5
Status	Udgivet - feb. 2022
Udgivet eksternt	Ja

Bibliografisk note

Funding Information:
Fundação de Amparo à Pesquisa do Estado de São Paulo—FAPESP (Grants 2013/08166–5 and 2018/15230–5). National Council for Scientific and Technological Development. CNPq (Grants 465259/2014–6 and 457733/2014–4). Coordination for the Improvement of Higher Education Personnel (CAPES). The National Institute of Science and Technology Complex Fluids (INCT-FCx) and NAP-FCx (Núcleo de Apoio à Pesquisa de Fluidos Complexos da Universidade de São Paulo). CAPES: INCT -Institutos Nacionais de Ciência e Tecnologia (Proc. 88887.137085/2017–00). Projeto Biocomputacional/CAPES (proc. no. 23038.004630/2014–35).

Funding Information:
IMC, HC and FHF thanks to FAPESP (Proc. 2013/08166-5). IMC thanks the National Council for Scientific and Technological Development (CNPq – 465259/2014-6). IMC and HC thank the Coordination for the Improvement of Higher Education Personnel (CAPES), the National Institute of Science and Technology Complex Fluids (INCT-FCx) and NAP-FCx (Núcleo de Apoio à Pesquisa de Fluidos Complexos da Universidade de São Paulo). G.P.B.Carretero thanks the Programa CAPES: INCT -Institutos Nacionais de Ciência e Tecnologia (Proc. 88887.137085/2017-00) and FAPESP (2018/15230-5). FHF thanks CNPq (Universal 457733/2014-4), and GKVS acknowledges the Projeto Biocomputacional/CAPES (proc. no. 23038.004630/2014-35) and CNPq (proc. 457733/2014-4).

Emneord

LCST
PDMAEMA-b-polystyrene
Polymersome
RAFT

Link til dokument

10.1007/s00289-020-03533-5

Citer dette

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title = "Stimuli-responsive polymersomes of poly [2-(dimethylamino) ethyl methacrylate]-b-polystyrene",

abstract = "Amphiphilic diblock copolymers may assemble in aqueous solutions to form vesicles delimited by a polymeric double layer, also known as polymersomes, considered a more robust option to liposomes. Diblock copolymers may respond to pH, temperature, and other conditions. Because of such properties, polymersomes are currently being studied as drug delivery systems or as nanoreactors. pH-responsive polymersomes are potentially crucial because unusual pH gradients are present in cells under several physiological and pathological conditions. We synthesized two diblock copolymers of poly [2-(dimethylamino) ethyl methacrylate]-block-polystyrene (PDMAEMA-b-PS) via RAFT. We both developed new materials and better-understood polymersomes' properties with pH and temperature-responsive groups with these polymers. GPC, 1H-NMR, and FTIR characterized copolymers. The ionization equilibrium of the PDMAEMA amino groups on the polymersomes was analyzed by potentiometric titration and Zeta potential measurements. The hydrodynamic radius of the polymersomes in different pH and temperatures was analyzed by DLS. Entrapment of an electron paramagnetic resonance probe indicated the presence of a hydrophilic inner core. Negative staining transmission electron microscopy showed spherical aggregates and confirmed the diameter around 80 nm. These polymersomes with dual stimulus–response (i.e., pH and temperature) may be a platform for gene delivery and nanoreactors.",

keywords = "LCST, PDMAEMA-b-polystyrene, Polymersome, RAFT, LCST, PDMAEMA-b-polystyrene, Polymersome, RAFT",

author = "{de Souza}, {Valdomiro V.} and Carretero, {Gustavo P.B.} and Vitale, {Phelipe A.M.} and {\'I}ris Todeschini and Kotani, {Paloma O.} and Saraiva, {Greice K.V.} and Guzzo, {Cristiane R.} and Hernan Chaimovich and Florenzano, {Fabio H.} and Cuccovia, {Iolanda M.}",

note = "Funding Information: Funda{\c c}{\~a}o de Amparo {\`a} Pesquisa do Estado de S{\~a}o Paulo—FAPESP (Grants 2013/08166–5 and 2018/15230–5). National Council for Scientific and Technological Development. CNPq (Grants 465259/2014–6 and 457733/2014–4). Coordination for the Improvement of Higher Education Personnel (CAPES). The National Institute of Science and Technology Complex Fluids (INCT-FCx) and NAP-FCx (N{\'u}cleo de Apoio {\`a} Pesquisa de Fluidos Complexos da Universidade de S{\~a}o Paulo). CAPES: INCT -Institutos Nacionais de Ci{\^e}ncia e Tecnologia (Proc. 88887.137085/2017–00). Projeto Biocomputacional/CAPES (proc. no. 23038.004630/2014–35). Funding Information: IMC, HC and FHF thanks to FAPESP (Proc. 2013/08166-5). IMC thanks the National Council for Scientific and Technological Development (CNPq – 465259/2014-6). IMC and HC thank the Coordination for the Improvement of Higher Education Personnel (CAPES), the National Institute of Science and Technology Complex Fluids (INCT-FCx) and NAP-FCx (N{\'u}cleo de Apoio {\`a} Pesquisa de Fluidos Complexos da Universidade de S{\~a}o Paulo). G.P.B.Carretero thanks the Programa CAPES: INCT -Institutos Nacionais de Ci{\^e}ncia e Tecnologia (Proc. 88887.137085/2017-00) and FAPESP (2018/15230-5). FHF thanks CNPq (Universal 457733/2014-4), and GKVS acknowledges the Projeto Biocomputacional/CAPES (proc. no. 23038.004630/2014-35) and CNPq (proc. 457733/2014-4). ",

year = "2022",

month = feb,

doi = "10.1007/s00289-020-03533-5",

language = "English",

volume = "79",

pages = "785--805",

journal = "Polymer Bulletin",

issn = "0170-0839",

number = "2",

}

TY - JOUR

T1 - Stimuli-responsive polymersomes of poly [2-(dimethylamino) ethyl methacrylate]-b-polystyrene

AU - de Souza, Valdomiro V.

AU - Carretero, Gustavo P.B.

AU - Vitale, Phelipe A.M.

AU - Todeschini, Íris

AU - Kotani, Paloma O.

AU - Saraiva, Greice K.V.

AU - Guzzo, Cristiane R.

AU - Chaimovich, Hernan

AU - Florenzano, Fabio H.

AU - Cuccovia, Iolanda M.

N1 - Funding Information: Fundação de Amparo à Pesquisa do Estado de São Paulo—FAPESP (Grants 2013/08166–5 and 2018/15230–5). National Council for Scientific and Technological Development. CNPq (Grants 465259/2014–6 and 457733/2014–4). Coordination for the Improvement of Higher Education Personnel (CAPES). The National Institute of Science and Technology Complex Fluids (INCT-FCx) and NAP-FCx (Núcleo de Apoio à Pesquisa de Fluidos Complexos da Universidade de São Paulo). CAPES: INCT -Institutos Nacionais de Ciência e Tecnologia (Proc. 88887.137085/2017–00). Projeto Biocomputacional/CAPES (proc. no. 23038.004630/2014–35). Funding Information: IMC, HC and FHF thanks to FAPESP (Proc. 2013/08166-5). IMC thanks the National Council for Scientific and Technological Development (CNPq – 465259/2014-6). IMC and HC thank the Coordination for the Improvement of Higher Education Personnel (CAPES), the National Institute of Science and Technology Complex Fluids (INCT-FCx) and NAP-FCx (Núcleo de Apoio à Pesquisa de Fluidos Complexos da Universidade de São Paulo). G.P.B.Carretero thanks the Programa CAPES: INCT -Institutos Nacionais de Ciência e Tecnologia (Proc. 88887.137085/2017-00) and FAPESP (2018/15230-5). FHF thanks CNPq (Universal 457733/2014-4), and GKVS acknowledges the Projeto Biocomputacional/CAPES (proc. no. 23038.004630/2014-35) and CNPq (proc. 457733/2014-4).

PY - 2022/2

Y1 - 2022/2

N2 - Amphiphilic diblock copolymers may assemble in aqueous solutions to form vesicles delimited by a polymeric double layer, also known as polymersomes, considered a more robust option to liposomes. Diblock copolymers may respond to pH, temperature, and other conditions. Because of such properties, polymersomes are currently being studied as drug delivery systems or as nanoreactors. pH-responsive polymersomes are potentially crucial because unusual pH gradients are present in cells under several physiological and pathological conditions. We synthesized two diblock copolymers of poly [2-(dimethylamino) ethyl methacrylate]-block-polystyrene (PDMAEMA-b-PS) via RAFT. We both developed new materials and better-understood polymersomes' properties with pH and temperature-responsive groups with these polymers. GPC, 1H-NMR, and FTIR characterized copolymers. The ionization equilibrium of the PDMAEMA amino groups on the polymersomes was analyzed by potentiometric titration and Zeta potential measurements. The hydrodynamic radius of the polymersomes in different pH and temperatures was analyzed by DLS. Entrapment of an electron paramagnetic resonance probe indicated the presence of a hydrophilic inner core. Negative staining transmission electron microscopy showed spherical aggregates and confirmed the diameter around 80 nm. These polymersomes with dual stimulus–response (i.e., pH and temperature) may be a platform for gene delivery and nanoreactors.

AB - Amphiphilic diblock copolymers may assemble in aqueous solutions to form vesicles delimited by a polymeric double layer, also known as polymersomes, considered a more robust option to liposomes. Diblock copolymers may respond to pH, temperature, and other conditions. Because of such properties, polymersomes are currently being studied as drug delivery systems or as nanoreactors. pH-responsive polymersomes are potentially crucial because unusual pH gradients are present in cells under several physiological and pathological conditions. We synthesized two diblock copolymers of poly [2-(dimethylamino) ethyl methacrylate]-block-polystyrene (PDMAEMA-b-PS) via RAFT. We both developed new materials and better-understood polymersomes' properties with pH and temperature-responsive groups with these polymers. GPC, 1H-NMR, and FTIR characterized copolymers. The ionization equilibrium of the PDMAEMA amino groups on the polymersomes was analyzed by potentiometric titration and Zeta potential measurements. The hydrodynamic radius of the polymersomes in different pH and temperatures was analyzed by DLS. Entrapment of an electron paramagnetic resonance probe indicated the presence of a hydrophilic inner core. Negative staining transmission electron microscopy showed spherical aggregates and confirmed the diameter around 80 nm. These polymersomes with dual stimulus–response (i.e., pH and temperature) may be a platform for gene delivery and nanoreactors.

KW - LCST

KW - PDMAEMA-b-polystyrene

KW - Polymersome

KW - RAFT

KW - LCST

KW - PDMAEMA-b-polystyrene

KW - Polymersome

KW - RAFT

U2 - 10.1007/s00289-020-03533-5

DO - 10.1007/s00289-020-03533-5

M3 - Journal article

AN - SCOPUS:85119060440

SN - 0170-0839

VL - 79

SP - 785

EP - 805

JO - Polymer Bulletin

JF - Polymer Bulletin

IS - 2

ER -