Spatial distribution of Escherichia coli in the mouse large intestine inferred from rRNA in situ hybridization.

Lars K Poulsen, Fusheng Lan, Claus S Kristensen, Palle Hobolth, Soren Molin, Karen A Krogfelt

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review


Fluorescent oligonucleotide probes targeting rRNA were used to develop an in situ hybridization technique by which the spatial distribution of Escherichia coli in the large intestines of streptomycin-treated mice was determined. Single E. coli cells were identified in thin frozen sections from the large intestines by the use of a probe specific for E. coli 23S rRNA. Furthermore, the total bacterial population was visualized with an rRNA probe targeting the domain Bacteria. By this technique, all E. coli cells were seen embedded in the mucosal material overlying the epithelial cells of the large intestine, and no direct attachment to the epithelium was observed.
TidsskriftInfection and Immunity
Udgave nummer11
Sider (fra-til)5191-5194
Antal sider4
StatusUdgivet - 1994
Udgivet eksterntJa

Citer dette