Sequence and context dependence of EF-hand loop dynamics. An textlesssuptextgreater15textless/suptextgreaterN relaxation study of a calcium-binding site mutant of calbindin D(9k)

A. Malmendal, G. Carlström, C. Hambraeus, T. Drakenberg, S. Forsén, M. Akke

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

The influence of amino acid sequence and structural context on the backbone dynamics of EF-hand calcium-binding loops was investigated using 15N spin relaxation measurements on the calcium-free state of the calbindin D(9k) mutant (A14D+A15$+P20$+N21G+P43M), in which the N-terminal pseudo- EF-hand loop, characteristic of S100 proteins, was engineered so as to conform with the C-terminal consensus EF-hand loop. The results were compared to a previous study of the apo state of the wild-type-like P43G calbindin D(9k) mutant. In the helical regions, the agreement with the P43G data is excellent, indicating that the structure and dynamics of the protein core are unaffected by the substitutions in the N-terminal loop. In the calcium- binding loops, the flexibility is drastically decreased compared to P43G, with the modified N-terminal loop showing a motional restriction comparable to that of the surrounding helixes. As in P43G, the motions in the C-terminal loop are less restricted than in the N-terminal loop. Differences in key hydrogen-bonding interactions correlate well with differences in dynamics and offer insights into the relationship between structure and dynamics of these EF-hand loops. It appears that the entire N-terminal EF-hand is built to form a rigid structure that allows calcium binding with only minor rearrangements and that the structural and dynamical properties of the entire EF-hand- rather than the loop sequence per se-is the major determinant of loop flexibility in this system.
OriginalsprogEngelsk
TidsskriftBiochemistry
Vol/bind37
Udgave nummer8
ISSN0006-2960
DOI
StatusUdgivet - 1998

Citer dette

@article{ea854c5a297b48079692dff352df6a5e,
title = "Sequence and context dependence of EF-hand loop dynamics. An textlesssuptextgreater15textless/suptextgreaterN relaxation study of a calcium-binding site mutant of calbindin D(9k)",
abstract = "The influence of amino acid sequence and structural context on the backbone dynamics of EF-hand calcium-binding loops was investigated using 15N spin relaxation measurements on the calcium-free state of the calbindin D(9k) mutant (A14D+A15$+P20$+N21G+P43M), in which the N-terminal pseudo- EF-hand loop, characteristic of S100 proteins, was engineered so as to conform with the C-terminal consensus EF-hand loop. The results were compared to a previous study of the apo state of the wild-type-like P43G calbindin D(9k) mutant. In the helical regions, the agreement with the P43G data is excellent, indicating that the structure and dynamics of the protein core are unaffected by the substitutions in the N-terminal loop. In the calcium- binding loops, the flexibility is drastically decreased compared to P43G, with the modified N-terminal loop showing a motional restriction comparable to that of the surrounding helixes. As in P43G, the motions in the C-terminal loop are less restricted than in the N-terminal loop. Differences in key hydrogen-bonding interactions correlate well with differences in dynamics and offer insights into the relationship between structure and dynamics of these EF-hand loops. It appears that the entire N-terminal EF-hand is built to form a rigid structure that allows calcium binding with only minor rearrangements and that the structural and dynamical properties of the entire EF-hand- rather than the loop sequence per se-is the major determinant of loop flexibility in this system.",
author = "A. Malmendal and G. Carlstr{\"o}m and C. Hambraeus and T. Drakenberg and S. Fors{\'e}n and M. Akke",
year = "1998",
doi = "10.1021/bi971798a",
language = "English",
volume = "37",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "8",

}

Sequence and context dependence of EF-hand loop dynamics. An textlesssuptextgreater15textless/suptextgreaterN relaxation study of a calcium-binding site mutant of calbindin D(9k). / Malmendal, A.; Carlström, G.; Hambraeus, C.; Drakenberg, T.; Forsén, S.; Akke, M.

I: Biochemistry, Bind 37, Nr. 8, 1998.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Sequence and context dependence of EF-hand loop dynamics. An textlesssuptextgreater15textless/suptextgreaterN relaxation study of a calcium-binding site mutant of calbindin D(9k)

AU - Malmendal, A.

AU - Carlström, G.

AU - Hambraeus, C.

AU - Drakenberg, T.

AU - Forsén, S.

AU - Akke, M.

PY - 1998

Y1 - 1998

N2 - The influence of amino acid sequence and structural context on the backbone dynamics of EF-hand calcium-binding loops was investigated using 15N spin relaxation measurements on the calcium-free state of the calbindin D(9k) mutant (A14D+A15$+P20$+N21G+P43M), in which the N-terminal pseudo- EF-hand loop, characteristic of S100 proteins, was engineered so as to conform with the C-terminal consensus EF-hand loop. The results were compared to a previous study of the apo state of the wild-type-like P43G calbindin D(9k) mutant. In the helical regions, the agreement with the P43G data is excellent, indicating that the structure and dynamics of the protein core are unaffected by the substitutions in the N-terminal loop. In the calcium- binding loops, the flexibility is drastically decreased compared to P43G, with the modified N-terminal loop showing a motional restriction comparable to that of the surrounding helixes. As in P43G, the motions in the C-terminal loop are less restricted than in the N-terminal loop. Differences in key hydrogen-bonding interactions correlate well with differences in dynamics and offer insights into the relationship between structure and dynamics of these EF-hand loops. It appears that the entire N-terminal EF-hand is built to form a rigid structure that allows calcium binding with only minor rearrangements and that the structural and dynamical properties of the entire EF-hand- rather than the loop sequence per se-is the major determinant of loop flexibility in this system.

AB - The influence of amino acid sequence and structural context on the backbone dynamics of EF-hand calcium-binding loops was investigated using 15N spin relaxation measurements on the calcium-free state of the calbindin D(9k) mutant (A14D+A15$+P20$+N21G+P43M), in which the N-terminal pseudo- EF-hand loop, characteristic of S100 proteins, was engineered so as to conform with the C-terminal consensus EF-hand loop. The results were compared to a previous study of the apo state of the wild-type-like P43G calbindin D(9k) mutant. In the helical regions, the agreement with the P43G data is excellent, indicating that the structure and dynamics of the protein core are unaffected by the substitutions in the N-terminal loop. In the calcium- binding loops, the flexibility is drastically decreased compared to P43G, with the modified N-terminal loop showing a motional restriction comparable to that of the surrounding helixes. As in P43G, the motions in the C-terminal loop are less restricted than in the N-terminal loop. Differences in key hydrogen-bonding interactions correlate well with differences in dynamics and offer insights into the relationship between structure and dynamics of these EF-hand loops. It appears that the entire N-terminal EF-hand is built to form a rigid structure that allows calcium binding with only minor rearrangements and that the structural and dynamical properties of the entire EF-hand- rather than the loop sequence per se-is the major determinant of loop flexibility in this system.

U2 - 10.1021/bi971798a

DO - 10.1021/bi971798a

M3 - Journal article

VL - 37

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 8

ER -