Role of cutaneous surface fluid in frog osmoregulation

Erik Hviid Larsen, Hans Ramløv

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    Resumé

    The study investigated whether evaporative water loss (EWL) in frogs stems from water diffusing through the skin or fluid secreted by mucous glands. Osmolality of cutaneous surface fluid (CSF) of Rana esculenta (Pelophylax kl. esculentus) subjected to isoproterenol or 30 °C–34 °C was 191 ± 9.3 and 181 ± 7.5 mosm/kg, respectively, as compared to lymph osmolality of, 249 ± 10 mosm/kg. Cation concentrations of CSF were likewise independent of pre-treatment with averages of, [Na+] = 65.5 ± 5.1 and [K+] = 14.9 ± 1.6 mmol/L, and lymph concentrations of 116 mmol Na+/L and 5.1 mmol K+/L. The relatively high [K+] confirms that CSF is produced by submucosal glands. Since the chemical energy of water of CSF was always higher than that of body fluids, diffusion of water would be from CSF to the interstitial fluid and not in the opposite direction. It is concluded that volume and composition of CSF are regulated by subepidermal exocrine gland secretion balanced by EWL into the atmosphere and ion reuptake by the epidermal epithelium. Previously discovered regulatory mechanisms of epithelial ion absorption, hitherto not ascribed a body function, fit well with a role in regulating turnover of CSF. As a regulated external physiological compartment, CSF would be of importance for the immune defenses that amphibians employ in protecting their skin.
    OriginalsprogEngelsk
    TidsskriftComparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology
    Vol/bind165
    Udgave nummer3
    Sider (fra-til)365-370
    Antal sider6
    ISSN1095-6433
    DOI
    StatusUdgivet - 2013

    Citer dette

    @article{507c607c9c5947758d64c61f0f2ddf0d,
    title = "Role of cutaneous surface fluid in frog osmoregulation",
    abstract = "The study investigated whether evaporative water loss (EWL) in frogs stems from water diffusing through the skin or fluid secreted by mucous glands. Osmolality of cutaneous surface fluid (CSF) of Rana esculenta (Pelophylax kl. esculentus) subjected to isoproterenol or 30 °C–34 °C was 191 ± 9.3 and 181 ± 7.5 mosm/kg, respectively, as compared to lymph osmolality of, 249 ± 10 mosm/kg. Cation concentrations of CSF were likewise independent of pre-treatment with averages of, [Na+] = 65.5 ± 5.1 and [K+] = 14.9 ± 1.6 mmol/L, and lymph concentrations of 116 mmol Na+/L and 5.1 mmol K+/L. The relatively high [K+] confirms that CSF is produced by submucosal glands. Since the chemical energy of water of CSF was always higher than that of body fluids, diffusion of water would be from CSF to the interstitial fluid and not in the opposite direction. It is concluded that volume and composition of CSF are regulated by subepidermal exocrine gland secretion balanced by EWL into the atmosphere and ion reuptake by the epidermal epithelium. Previously discovered regulatory mechanisms of epithelial ion absorption, hitherto not ascribed a body function, fit well with a role in regulating turnover of CSF. As a regulated external physiological compartment, CSF would be of importance for the immune defenses that amphibians employ in protecting their skin.",
    keywords = "evaporative water loss in the frog, subepidermal mucous glands, water and ion turnover of cutaneous surface fluid in frog, chytridiomycosis",
    author = "Larsen, {Erik Hviid} and Hans Raml{\o}v",
    year = "2013",
    doi = "10.1016/j.cbpa.2013.04.005",
    language = "English",
    volume = "165",
    pages = "365--370",
    journal = "Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology",
    issn = "1095-6433",
    publisher = "Pergamon Press",
    number = "3",

    }

    Role of cutaneous surface fluid in frog osmoregulation. / Larsen, Erik Hviid; Ramløv, Hans.

    I: Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology, Bind 165, Nr. 3, 2013, s. 365-370.

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    TY - JOUR

    T1 - Role of cutaneous surface fluid in frog osmoregulation

    AU - Larsen, Erik Hviid

    AU - Ramløv, Hans

    PY - 2013

    Y1 - 2013

    N2 - The study investigated whether evaporative water loss (EWL) in frogs stems from water diffusing through the skin or fluid secreted by mucous glands. Osmolality of cutaneous surface fluid (CSF) of Rana esculenta (Pelophylax kl. esculentus) subjected to isoproterenol or 30 °C–34 °C was 191 ± 9.3 and 181 ± 7.5 mosm/kg, respectively, as compared to lymph osmolality of, 249 ± 10 mosm/kg. Cation concentrations of CSF were likewise independent of pre-treatment with averages of, [Na+] = 65.5 ± 5.1 and [K+] = 14.9 ± 1.6 mmol/L, and lymph concentrations of 116 mmol Na+/L and 5.1 mmol K+/L. The relatively high [K+] confirms that CSF is produced by submucosal glands. Since the chemical energy of water of CSF was always higher than that of body fluids, diffusion of water would be from CSF to the interstitial fluid and not in the opposite direction. It is concluded that volume and composition of CSF are regulated by subepidermal exocrine gland secretion balanced by EWL into the atmosphere and ion reuptake by the epidermal epithelium. Previously discovered regulatory mechanisms of epithelial ion absorption, hitherto not ascribed a body function, fit well with a role in regulating turnover of CSF. As a regulated external physiological compartment, CSF would be of importance for the immune defenses that amphibians employ in protecting their skin.

    AB - The study investigated whether evaporative water loss (EWL) in frogs stems from water diffusing through the skin or fluid secreted by mucous glands. Osmolality of cutaneous surface fluid (CSF) of Rana esculenta (Pelophylax kl. esculentus) subjected to isoproterenol or 30 °C–34 °C was 191 ± 9.3 and 181 ± 7.5 mosm/kg, respectively, as compared to lymph osmolality of, 249 ± 10 mosm/kg. Cation concentrations of CSF were likewise independent of pre-treatment with averages of, [Na+] = 65.5 ± 5.1 and [K+] = 14.9 ± 1.6 mmol/L, and lymph concentrations of 116 mmol Na+/L and 5.1 mmol K+/L. The relatively high [K+] confirms that CSF is produced by submucosal glands. Since the chemical energy of water of CSF was always higher than that of body fluids, diffusion of water would be from CSF to the interstitial fluid and not in the opposite direction. It is concluded that volume and composition of CSF are regulated by subepidermal exocrine gland secretion balanced by EWL into the atmosphere and ion reuptake by the epidermal epithelium. Previously discovered regulatory mechanisms of epithelial ion absorption, hitherto not ascribed a body function, fit well with a role in regulating turnover of CSF. As a regulated external physiological compartment, CSF would be of importance for the immune defenses that amphibians employ in protecting their skin.

    KW - evaporative water loss in the frog

    KW - subepidermal mucous glands

    KW - water and ion turnover of cutaneous surface fluid in frog

    KW - chytridiomycosis

    U2 - 10.1016/j.cbpa.2013.04.005

    DO - 10.1016/j.cbpa.2013.04.005

    M3 - Journal article

    VL - 165

    SP - 365

    EP - 370

    JO - Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology

    JF - Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology

    SN - 1095-6433

    IS - 3

    ER -