Replication termination without a replication fork trap

Elisa Galli, Jean-Luc Ferrat, Jean-Michel Desfontaines, Marie-Eve Val, Ole Skovgaard, Francois-Xavier Barre, Christophe Possoz

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Bacterial chromosomes harbour a unique origin of bidirectional replication, oriC. They are almost always circular, with replication terminating in a region diametrically opposite to oriC, the terminus. The oriC-terminus organisation is reflected by the orientation of the genes and by the disposition of DNA-binding protein motifs implicated in the coordination of chromosome replication and segregation with cell division. Correspondingly, the E. coli and B. subtilis model bacteria possess a replication fork trap system, Tus/ter and RTP/ter, respectively, which enforces replication termination in the terminus region. Here, we show that tus and rtp are restricted to four clades of bacteria, suggesting that tus was recently domesticated from a plasmid gene. We further demonstrate that there is no replication fork system in Vibrio cholerae, a bacterium closely related to E. coli. Marker frequency analysis showed that replication forks originating from ectopic origins were not blocked in the terminus region of either of the two V. cholerae chromosomes, but progressed normally until they encountered an opposite fork. As expected, termination synchrony of the two chromosomes is disrupted by these ectopic origins. Finally, we show that premature completion of the primary chromosome replication did not modify the choreography of segregation of its terminus region.
OriginalsprogEngelsk
Artikelnummer8315
TidsskriftScientific Reports
Vol/bind2019
Udgave nummer9
Antal sider11
ISSN2045-2322
DOI
StatusUdgivet - 5 jun. 2019

Citer dette

Galli, E., Ferrat, J-L., Desfontaines, J-M., Val, M-E., Skovgaard, O., Barre, F-X., & Possoz, C. (2019). Replication termination without a replication fork trap. Scientific Reports, 2019(9), [8315]. https://doi.org/10.1038/s41598-019-43795-2
Galli, Elisa ; Ferrat, Jean-Luc ; Desfontaines, Jean-Michel ; Val, Marie-Eve ; Skovgaard, Ole ; Barre, Francois-Xavier ; Possoz, Christophe. / Replication termination without a replication fork trap. I: Scientific Reports. 2019 ; Bind 2019, Nr. 9.
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Galli, E, Ferrat, J-L, Desfontaines, J-M, Val, M-E, Skovgaard, O, Barre, F-X & Possoz, C 2019, 'Replication termination without a replication fork trap' Scientific Reports, bind 2019, nr. 9, 8315. https://doi.org/10.1038/s41598-019-43795-2

Replication termination without a replication fork trap. / Galli, Elisa; Ferrat, Jean-Luc; Desfontaines, Jean-Michel; Val, Marie-Eve; Skovgaard, Ole; Barre, Francois-Xavier; Possoz, Christophe.

I: Scientific Reports, Bind 2019, Nr. 9, 8315, 05.06.2019.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Replication termination without a replication fork trap

AU - Galli, Elisa

AU - Ferrat, Jean-Luc

AU - Desfontaines, Jean-Michel

AU - Val, Marie-Eve

AU - Skovgaard, Ole

AU - Barre, Francois-Xavier

AU - Possoz, Christophe

PY - 2019/6/5

Y1 - 2019/6/5

N2 - Bacterial chromosomes harbour a unique origin of bidirectional replication, oriC. They are almost always circular, with replication terminating in a region diametrically opposite to oriC, the terminus. The oriC-terminus organisation is reflected by the orientation of the genes and by the disposition of DNA-binding protein motifs implicated in the coordination of chromosome replication and segregation with cell division. Correspondingly, the E. coli and B. subtilis model bacteria possess a replication fork trap system, Tus/ter and RTP/ter, respectively, which enforces replication termination in the terminus region. Here, we show that tus and rtp are restricted to four clades of bacteria, suggesting that tus was recently domesticated from a plasmid gene. We further demonstrate that there is no replication fork system in Vibrio cholerae, a bacterium closely related to E. coli. Marker frequency analysis showed that replication forks originating from ectopic origins were not blocked in the terminus region of either of the two V. cholerae chromosomes, but progressed normally until they encountered an opposite fork. As expected, termination synchrony of the two chromosomes is disrupted by these ectopic origins. Finally, we show that premature completion of the primary chromosome replication did not modify the choreography of segregation of its terminus region.

AB - Bacterial chromosomes harbour a unique origin of bidirectional replication, oriC. They are almost always circular, with replication terminating in a region diametrically opposite to oriC, the terminus. The oriC-terminus organisation is reflected by the orientation of the genes and by the disposition of DNA-binding protein motifs implicated in the coordination of chromosome replication and segregation with cell division. Correspondingly, the E. coli and B. subtilis model bacteria possess a replication fork trap system, Tus/ter and RTP/ter, respectively, which enforces replication termination in the terminus region. Here, we show that tus and rtp are restricted to four clades of bacteria, suggesting that tus was recently domesticated from a plasmid gene. We further demonstrate that there is no replication fork system in Vibrio cholerae, a bacterium closely related to E. coli. Marker frequency analysis showed that replication forks originating from ectopic origins were not blocked in the terminus region of either of the two V. cholerae chromosomes, but progressed normally until they encountered an opposite fork. As expected, termination synchrony of the two chromosomes is disrupted by these ectopic origins. Finally, we show that premature completion of the primary chromosome replication did not modify the choreography of segregation of its terminus region.

U2 - 10.1038/s41598-019-43795-2

DO - 10.1038/s41598-019-43795-2

M3 - Journal article

VL - 2019

JO - Scientific Reports

JF - Scientific Reports

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Galli E, Ferrat J-L, Desfontaines J-M, Val M-E, Skovgaard O, Barre F-X et al. Replication termination without a replication fork trap. Scientific Reports. 2019 jun 5;2019(9). 8315. https://doi.org/10.1038/s41598-019-43795-2