Henning F. Bjerregaard, Roskilde University, Department of Science, Systems and Models, 4000 Roskilde, Denmark. HFB@ RUC.DK
Reactive oxygen species (ROS) like, hydrogen peroxide (H2O2) has traditionally been regarded as toxic by-products of aerobic metabolism. However, recent findings indicate that H2O2 act as a signalling molecule. The aim of the present study was to monitor, in real time, the rates of ROS generation in order to directly determine their production dynamics in living cells in response to hormonal signal events in the A6 cell culture. A6 cells have a divalent cation-sensing receptor (the extracellular calcium receptor) that can be stimulated with cadmium (Cd) and thereby induce a fast and transient liberation of calcium from intracellular stores, due to G-protein stimulation of phospholipase C and release of inositol -3 phosphate. Cd (0.4 mM) treatment of A6 cells enhanced the ROS production after one minutes incubation. The production rate was constant for at least 10 to 20 min. Experiments showed that the Cd induced increase in ROS production was inhibited by buffering of intracellular calcium with BAPTA, by the antioxidant N-acetylcysteine and by uncoupling of mitochondrial oxidative phosphorylation from respiration with CCCP. These results indicate that Cd generate a prompt initiation of ROS production from mitochondria due to an increase in the intracellular calcium concentration.
|Status||Udgivet - 2009|
|Begivenhed||36 th International Congress of Physiological Sciences - Kyoto, Japan|
Varighed: 27 jul. 2009 → 1 aug. 2009
|Konference||36 th International Congress of Physiological Sciences|
|Periode||27/07/2009 → 01/08/2009|