Regulation of lactase-phlorizin hydrolase gene expression by the caudal-related homoeodomain protein Cdx-2

Jesper T. Troelsen*, Cathy Mitchelmore, Nikolaj Spodsberg, Anette M. Jensen, Ove Noren, Hans Sjöström

*Corresponding author

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review


Lactase-phlorizin hydrolase is exclusively expressed in the small intestine and is often used as a marker for the differentiation of enterocytes. The cis-element CE-LPH1 found in the lactase-phlorizin hydrolase promoter has previously been shown to bind an intestinal-specific nuclear factor. By electrophoretic mobility-shift assay it was shown that the factor Cdx-2 (a homoeodomain-protein related to caudal) binds to a TTTAC sequence in the CE-LPH1. Furthermore it was demonstrated that Cdx-2 is able to activate reporter gene transcription by binding to CE-LPH1. A mutation in CE-LPH1, which does not affect Cdx-2 binding, results in a higher transcriptional activity, indicating that the CE-LPH1 site contains other binding site(s) in addition to the Cdx-2-binding site.
TidsskriftBiochemical Journal
Udgave nummer3
Sider (fra-til)833-838
Antal sider6
StatusUdgivet - 15 mar. 1997
Udgivet eksterntJa

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