Recrystallization in a Freezing Tolerant Antarctic Nematode, Panagrolaimus davidi, and a Alpine Weta, Hemideina maori (Orthoptera; Stenopelmatidae)

Hans Ramløv, David A. Wharton, Peter W. Wilson

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    Resumé

    The ability of haemolymph from the freezing tolerant weta,Hemideina maori,and supernatant from homogenates of the freezing tolerant nematodePanagrolaimus davidito inhibit the recrystallization of ice was examined using the “splat freezing” technique and annealing on a cryomicroscope stage. There was no recrystallization inhibition in weta haemolymph or in insect ringer controls. Recrystallization inhibition was present in the nematode supernatant but this was destroyed by heating and was absent in controls.P. davidisurvives intracellular freezing and recrystallization inhibition may be important for the survival of this stress.
    OriginalsprogEngelsk
    TidsskriftCryobiology
    Vol/bind33
    Udgave nummer6
    Sider (fra-til)607-613
    ISSN0011-2240
    DOI
    StatusUdgivet - 1996

    Citer dette

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    title = "Recrystallization in a Freezing Tolerant Antarctic Nematode, Panagrolaimus davidi, and a Alpine Weta, Hemideina maori (Orthoptera; Stenopelmatidae)",
    abstract = "The ability of haemolymph from the freezing tolerant weta,Hemideina maori,and supernatant from homogenates of the freezing tolerant nematodePanagrolaimus davidito inhibit the recrystallization of ice was examined using the “splat freezing” technique and annealing on a cryomicroscope stage. There was no recrystallization inhibition in weta haemolymph or in insect ringer controls. Recrystallization inhibition was present in the nematode supernatant but this was destroyed by heating and was absent in controls.P. davidisurvives intracellular freezing and recrystallization inhibition may be important for the survival of this stress.",
    author = "Hans Raml{\o}v and Wharton, {David A.} and Wilson, {Peter W.}",
    year = "1996",
    doi = "10.1006/cryo.1996.0064",
    language = "English",
    volume = "33",
    pages = "607--613",
    journal = "Cryobiology",
    issn = "0011-2240",
    publisher = "Academic Press",
    number = "6",

    }

    Recrystallization in a Freezing Tolerant Antarctic Nematode, Panagrolaimus davidi, and a Alpine Weta, Hemideina maori (Orthoptera; Stenopelmatidae). / Ramløv, Hans; Wharton, David A.; Wilson, Peter W.

    I: Cryobiology, Bind 33, Nr. 6, 1996, s. 607-613.

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    TY - JOUR

    T1 - Recrystallization in a Freezing Tolerant Antarctic Nematode, Panagrolaimus davidi, and a Alpine Weta, Hemideina maori (Orthoptera; Stenopelmatidae)

    AU - Ramløv, Hans

    AU - Wharton, David A.

    AU - Wilson, Peter W.

    PY - 1996

    Y1 - 1996

    N2 - The ability of haemolymph from the freezing tolerant weta,Hemideina maori,and supernatant from homogenates of the freezing tolerant nematodePanagrolaimus davidito inhibit the recrystallization of ice was examined using the “splat freezing” technique and annealing on a cryomicroscope stage. There was no recrystallization inhibition in weta haemolymph or in insect ringer controls. Recrystallization inhibition was present in the nematode supernatant but this was destroyed by heating and was absent in controls.P. davidisurvives intracellular freezing and recrystallization inhibition may be important for the survival of this stress.

    AB - The ability of haemolymph from the freezing tolerant weta,Hemideina maori,and supernatant from homogenates of the freezing tolerant nematodePanagrolaimus davidito inhibit the recrystallization of ice was examined using the “splat freezing” technique and annealing on a cryomicroscope stage. There was no recrystallization inhibition in weta haemolymph or in insect ringer controls. Recrystallization inhibition was present in the nematode supernatant but this was destroyed by heating and was absent in controls.P. davidisurvives intracellular freezing and recrystallization inhibition may be important for the survival of this stress.

    U2 - 10.1006/cryo.1996.0064

    DO - 10.1006/cryo.1996.0064

    M3 - Journal article

    VL - 33

    SP - 607

    EP - 613

    JO - Cryobiology

    JF - Cryobiology

    SN - 0011-2240

    IS - 6

    ER -