Quantifying spatial position in a branched structure in immunostained mouse tissue sections

Silja Heilmann, Henrik Semb, Pia Nyeng

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review


We have developed a protocol to quantify the position of a cell in a branched structure based on two-dimensional microscopy images of tissue sections. Biological branched structures include organs such as the lungs, kidneys, and pancreas. In these organs, cell fate has been correlated with position, based on a qualitative estimate. However, a quantitative means of evaluating the cell position has been lacking. With this protocol, the correlation between cell fate and cell position was measured in mouse embryonic pancreas.
TidsskriftSTAR protocols
StatusUdgivet - 1 okt. 2021

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