Quantifying spatial position in a branched structure in immunostained mouse tissue sections

Silja Heilmann; Henrik  Semb; Pia Nyeng

doi:10.1016/j.xpro.2021.100806

Quantifying spatial position in a branched structure in immunostained mouse tissue sections

Silja Heilmann, Henrik Semb, Pia Nyeng^*

^*Corresponding author

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Abstract

We have developed a protocol to quantify the position of a cell in a branched structure based on two-dimensional microscopy images of tissue sections. Biological branched structures include organs such as the lungs, kidneys, and pancreas. In these organs, cell fate has been correlated with position, based on a qualitative estimate. However, a quantitative means of evaluating the cell position has been lacking. With this protocol, the correlation between cell fate and cell position was measured in mouse embryonic pancreas.

Originalsprog	Engelsk
Artikelnummer	100806
Tidsskrift	STAR protocols
Vol/bind	2
Udgave nummer	4
ISSN	2666-1667
DOI	https://doi.org/10.1016/j.xpro.2021.100806
Status	Udgivet - 1 okt. 2021

Emneord

Bioinformatics
Cell Biology
Microscopy

Link til dokument

10.1016/j.xpro.2021.100806Licens: CC BY-NC-ND

Quantifying spatial position in a branched structure in immunostained mouse tissue sectionsForlagets udgivne version, 5,3 MBLicens: CC BY-NC-ND

Citer dette

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KW - Microscopy

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