Quantifying spatial position in a branched structure in immunostained mouse tissue sections

Silja Heilmann, Henrik Semb, Pia Nyeng*

*Corresponding author

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review


We have developed a protocol to quantify the position of a cell in a branched structure based on two-dimensional microscopy images of tissue sections. Biological branched structures include organs such as the lungs, kidneys, and pancreas. In these organs, cell fate has been correlated with position, based on a qualitative estimate. However, a quantitative means of evaluating the cell position has been lacking. With this protocol, the correlation between cell fate and cell position was measured in mouse embryonic pancreas.
TidsskriftSTAR protocols
Udgave nummer4
StatusUdgivet - 1 okt. 2021

Bibliografisk note

Funding Information:
This work was supported by JDRF grant 3?2011-214 and DFF grant number 6110-00091B. The Novo Nordisk Foundation Center for Stem Cell Biology is supported by a Novo Nordisk Foundation grant number NNF17CC0027852.


  • Bioinformatics
  • Cell Biology
  • Microscopy

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