Purification and crystallization of the yeast elongation factor eEF2

René Jorgensen; Anne Carr-Schmid; Pedro A. Ortiz; Terri Goss Kinzy; Gregers Rom Andersen

doi:10.1107/S0907444902003001

Purification and crystallization of the yeast elongation factor eEF2

René Jorgensen, Anne Carr-Schmid, Pedro A. Ortiz, Terri Goss Kinzy, Gregers Rom Andersen^*

^*Corresponding author

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Abstract

Crystals of the Saccharomyces cerevisiae elongation factor 2 (eEF2) in complex with GDP were obtained with the vapour-diffusion technique after rapid purification from industrial yeast. The crystals diffract to 2.85 Å and belong to the space group P2₁2₁2₁. A yeast strain expressing a functional histidine-tagged eEF2 as the only form of the protein further allows facilitated purification of the factor for both structural and functional studies.

Originalsprog	Engelsk
Tidsskrift	Acta Crystallographica Section D: Biological Crystallography
Vol/bind	58
Udgave nummer	4
Sider (fra-til)	712-715
Antal sider	4
ISSN	0907-4449
DOI	https://doi.org/10.1107/S0907444902003001
Status	Udgivet - 2002
Udgivet eksternt	Ja

Link til dokument

10.1107/S0907444902003001

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Citer dette

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abstract = "Crystals of the Saccharomyces cerevisiae elongation factor 2 (eEF2) in complex with GDP were obtained with the vapour-diffusion technique after rapid purification from industrial yeast. The crystals diffract to 2.85 {\AA} and belong to the space group P212121. A yeast strain expressing a functional histidine-tagged eEF2 as the only form of the protein further allows facilitated purification of the factor for both structural and functional studies.",

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AU - Carr-Schmid, Anne

AU - Ortiz, Pedro A.

AU - Kinzy, Terri Goss

AU - Andersen, Gregers Rom

PY - 2002

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AB - Crystals of the Saccharomyces cerevisiae elongation factor 2 (eEF2) in complex with GDP were obtained with the vapour-diffusion technique after rapid purification from industrial yeast. The crystals diffract to 2.85 Å and belong to the space group P212121. A yeast strain expressing a functional histidine-tagged eEF2 as the only form of the protein further allows facilitated purification of the factor for both structural and functional studies.

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