Purification and crystallization of the yeast elongation factor eEF2

René Jorgensen, Anne Carr-Schmid, Pedro A. Ortiz, Terri Goss Kinzy, Gregers Rom Andersen*

*Corresponding author

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Crystals of the Saccharomyces cerevisiae elongation factor 2 (eEF2) in complex with GDP were obtained with the vapour-diffusion technique after rapid purification from industrial yeast. The crystals diffract to 2.85 Å and belong to the space group P212121. A yeast strain expressing a functional histidine-tagged eEF2 as the only form of the protein further allows facilitated purification of the factor for both structural and functional studies.

TidsskriftActa Crystallographica Section D: Biological Crystallography
Udgave nummer4
Sider (fra-til)712-715
Antal sider4
StatusUdgivet - 2002
Udgivet eksterntJa

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