TY - JOUR
T1 - Protracted elimination of gold nanoparticles from mouse liver
AU - Sadauskas, Evaldas
AU - Danscher, Gorm
AU - Stoltenberg, Meredin
AU - Vogel, Ulla
AU - Larsen, Agnete
AU - Wallin, Erik Håkan Richard
PY - 2009
Y1 - 2009
N2 - The present study aims at revealing the fate of 40-nm gold nanoparticles after intravenous injections. The gold nanoparticles were traced histochemically with light and transmission electron microscopy using autometallographic (AMG) staining, and the gold content in the liver was determined with inductively coupled plasma mass spectrometry (ICP-MS). Gold nanoparticles were identified in almost all Kupffer cells one day after the injection, but the fraction of gold-loaded cells gradually decreased to about one fifth after 6 months. Transmission electron microscopic analysis showed that the gold nanoparticles had accumulated inside the vesicular lysosome/endosome-like structures of the macrophages. At day 1, about 4.5‰ of the area of the liver sections was AMG-stained, after 1 month it had decreased to 0.7‰, and thereafter no further significant reduction was recorded. Because ICP-MS only showed a 9% fall in the gold content over the observed 6 months, the AMG finding of a significant reduction in the stained area of the liver sections and number of macrophages loaded with gold nanoparticles reveals that over time an increasing part of the total amount of gold nanoparticles in the liver is contained in fewer macrophages accumulated in growing clusters
AB - The present study aims at revealing the fate of 40-nm gold nanoparticles after intravenous injections. The gold nanoparticles were traced histochemically with light and transmission electron microscopy using autometallographic (AMG) staining, and the gold content in the liver was determined with inductively coupled plasma mass spectrometry (ICP-MS). Gold nanoparticles were identified in almost all Kupffer cells one day after the injection, but the fraction of gold-loaded cells gradually decreased to about one fifth after 6 months. Transmission electron microscopic analysis showed that the gold nanoparticles had accumulated inside the vesicular lysosome/endosome-like structures of the macrophages. At day 1, about 4.5‰ of the area of the liver sections was AMG-stained, after 1 month it had decreased to 0.7‰, and thereafter no further significant reduction was recorded. Because ICP-MS only showed a 9% fall in the gold content over the observed 6 months, the AMG finding of a significant reduction in the stained area of the liver sections and number of macrophages loaded with gold nanoparticles reveals that over time an increasing part of the total amount of gold nanoparticles in the liver is contained in fewer macrophages accumulated in growing clusters
KW - Gold Nanoparticles
KW - Autometallography
KW - Liver
KW - Protracted elimination
KW - Inductively coupled plasma mass spectrometry
U2 - 10.1016/j.nano.2008.11.002
DO - 10.1016/j.nano.2008.11.002
M3 - Journal article
SN - 1549-9634
VL - 5
SP - 162
EP - 169
JO - Nanomedicine: Nanotechnology, Biology and Medicine
JF - Nanomedicine: Nanotechnology, Biology and Medicine
IS - 2
ER -