Probiotics and the immunological response to infant vaccinations; a double-blind randomized controlled trial

Camilla Adler Sørensen , E. Fuglsang, Charlotte Sværke Jørgensen, R. P. Laursen, Anni Larnkjær, C. Mølgaard, C. Ritz, K.F. Michaelsen, H. Frokiaer, K. A. Krogfelt

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Abstract

Objectives
To examine the effect of a combination of probiotics on the antibody response to pneumococcal and pertussis vaccination in healthy Danish children, aged 8–14 months, at the time of starting day care. Moreover, the cytokine response to lipopolysaccharide of whole blood was assessed.

Methods
A total of 290 children were randomly allocated to receive a combination of Bifidobacterium animalis ssp. lactis and Lactobacillus rhamnosus GG daily for a 6-month intervention period, and blood samples were drawn at the start and end of the study. Specific antibody response towards Streptococcus pneumoniae serotypes and Bordetella pertussis toxin, as well as endotoxin-induced interleukin-6 (IL-6) and interferon-γ (IFN-γ) production in blood were analysed by Luminex and ELISA.

Results
There was no significant difference between the average individual changes from baseline to end of study in antibody concentrations for S. pneumoniae for both the probiotics (340.4% ± 11.2%) and the placebo group (382.9% ± 10.4%) (p 0.525), nor for B. pertussis toxin in the two groups (probiotics 190.1% ± 12.6% versus placebo 238.8% ± 1.1%, p 0.340). The average individual change in IL-6 concentration was significantly lower in the probiotics versus the placebo group (2.9% ± 10.3% versus 33.7% ± 9.0%, p 0.024), whereas there was no difference in IFN-γ concentration (0.0% ± 0.2% versus –0.2% ± 0.1%, p 0.279).

Conclusions
The probiotic intervention did not affect the antibody response against S. pneumoniae and B. pertussis toxin in healthy Danish children.
OriginalsprogEngelsk
TidsskriftClinical Microbiology and Infection
Vol/bind25
Udgave nummer4
Sider (fra-til)511.e1–511.e7
ISSN1198-743X
DOI
StatusUdgivet - 2019
Udgivet eksterntJa

Citer dette

Adler Sørensen , C., Fuglsang, E., Sværke Jørgensen, C., Laursen, R. P., Larnkjær, A., Mølgaard, C., Ritz, C., Michaelsen, K. F., Frokiaer, H., & Krogfelt, K. A. (2019). Probiotics and the immunological response to infant vaccinations; a double-blind randomized controlled trial. Clinical Microbiology and Infection, 25(4), 511.e1–511.e7. https://doi.org/10.1016/j.cmi.2018.07.031