Peptide meets membrane: Investigating peptide-lipid interactions using small-angle scattering techniques

Josefine Eilsø Nielsen, Vladimir Rosenov Koynarev, Reidar Lund*

*Corresponding author

Publikation: Bidrag til tidsskriftReviewpeer review

Abstract

Peptide–lipid interactions play an important role in defining the mode of action of drugs and the molecular mechanism associated with many diseases. Model membranes consisting of simple lipid mixtures mimicking real cell membranes can provide insight into the structural and dynamic aspects associated with these interactions. Small-angle scattering techniques based on X-rays and neutrons (SAXS/SANS) allow in situ determination of peptide partition and structural changes in lipid bilayers in vesicles with relatively high resolution between 1-100 nm. With advanced instrumentation, time-resolved SANS/SAXS can be used to track equilibrium and nonequilibrium processes such as lipid transport and morphological transitions to time scales down to a millisecond. In this review, we provide an overview of recent advances in the understanding of complex peptide–lipid membrane interactions using SAXS/SANS methods and model lipid membrane unilamellar vesicles. Particular attention will be given to the data analysis, possible pitfalls, and how to extract quantitative information using these techniques.

OriginalsprogEngelsk
Artikelnummer101709
TidsskriftCurrent Opinion in Colloid and Interface Science
Vol/bind66
ISSN1359-0294
DOI
StatusUdgivet - aug. 2023
Udgivet eksterntJa

Bibliografisk note

Funding Information:
The authors gratefully acknowledge funding from the Norwegian Research Council (Project no. 315666 ) and NordForsk (Project no. 82004 ) for financial support.

Publisher Copyright:
© 2023 The Authors

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