PARAFAC Modeling of Fluorescence Excitation-Emission Spectra of Fish Bile for Rapid En Route Screening of PAC Exposure

Jan H. Christensen, Giorgio Tomasi, Jakob Strand, Ole Andersen

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    Resumé

    Polycyclic aromatic compound (PAC) metabolites in fish bile can be used as biomarkers for recent environmental exposure to PACs. Here, a novel method for rapid screening of nonhydrolyzed fish bile is presented. The method is based on excitation-emission fluorescence spectroscopy combined with parallel factor analysis (PARAFAC) and may constitute an alternative to fixed wavelength fluorescence and synchronous fluorescence spectroscopy (M). PARAFAC was applied to excitation-emission matrices (EEMs) of bile samples of shorthorn sculpins and European eels collected in Greenland and Denmark. The EEMs were decomposed into a four-factor PARAFAC model. The comparison of the PARAFAC factors with the EEMs of PAC metabolites and amino acids suggests that two factors are related to PAC metabolites and two correspond to fluorescent residues of tryptophan and tyrosine in bile proteins. A new standardization procedure based on the mean of the scores for the biological factors was used to correct for feeding status and sample dilution and, upon such normalization, the score plots of PARAFAC factors showed a clear distinction between exposed and nonexposed fish. A good correlation was found between the factor scores and 1-hydroxypyrene equivalents determined by SFS for high contamination levels, whereas the sensitivity was better for the EEM method
    OriginalsprogEngelsk
    TidsskriftEnvironmental Science & Technology (Washington)
    Vol/bind43
    Udgave nummer12
    Sider (fra-til)4439-4445
    ISSN0013-936X
    DOI
    StatusUdgivet - 2009

    Citer dette

    Christensen, Jan H. ; Tomasi, Giorgio ; Strand, Jakob ; Andersen, Ole. / PARAFAC Modeling of Fluorescence Excitation-Emission Spectra of Fish Bile for Rapid En Route Screening of PAC Exposure. I: Environmental Science & Technology (Washington). 2009 ; Bind 43, Nr. 12. s. 4439-4445.
    @article{2f89b690f9fd11de89af000ea68e967b,
    title = "PARAFAC Modeling of Fluorescence Excitation-Emission Spectra of Fish Bile for Rapid En Route Screening of PAC Exposure",
    abstract = "Polycyclic aromatic compound (PAC) metabolites in fish bile can be used as biomarkers for recent environmental exposure to PACs. Here, a novel method for rapid screening of nonhydrolyzed fish bile is presented. The method is based on excitation-emission fluorescence spectroscopy combined with parallel factor analysis (PARAFAC) and may constitute an alternative to fixed wavelength fluorescence and synchronous fluorescence spectroscopy (M). PARAFAC was applied to excitation-emission matrices (EEMs) of bile samples of shorthorn sculpins and European eels collected in Greenland and Denmark. The EEMs were decomposed into a four-factor PARAFAC model. The comparison of the PARAFAC factors with the EEMs of PAC metabolites and amino acids suggests that two factors are related to PAC metabolites and two correspond to fluorescent residues of tryptophan and tyrosine in bile proteins. A new standardization procedure based on the mean of the scores for the biological factors was used to correct for feeding status and sample dilution and, upon such normalization, the score plots of PARAFAC factors showed a clear distinction between exposed and nonexposed fish. A good correlation was found between the factor scores and 1-hydroxypyrene equivalents determined by SFS for high contamination levels, whereas the sensitivity was better for the EEM method",
    author = "Christensen, {Jan H.} and Giorgio Tomasi and Jakob Strand and Ole Andersen",
    year = "2009",
    doi = "10.1021/es900187x",
    language = "English",
    volume = "43",
    pages = "4439--4445",
    journal = "Environmental Science & Technology (Washington)",
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    PARAFAC Modeling of Fluorescence Excitation-Emission Spectra of Fish Bile for Rapid En Route Screening of PAC Exposure. / Christensen, Jan H.; Tomasi, Giorgio; Strand, Jakob; Andersen, Ole.

    I: Environmental Science & Technology (Washington), Bind 43, Nr. 12, 2009, s. 4439-4445.

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    TY - JOUR

    T1 - PARAFAC Modeling of Fluorescence Excitation-Emission Spectra of Fish Bile for Rapid En Route Screening of PAC Exposure

    AU - Christensen, Jan H.

    AU - Tomasi, Giorgio

    AU - Strand, Jakob

    AU - Andersen, Ole

    PY - 2009

    Y1 - 2009

    N2 - Polycyclic aromatic compound (PAC) metabolites in fish bile can be used as biomarkers for recent environmental exposure to PACs. Here, a novel method for rapid screening of nonhydrolyzed fish bile is presented. The method is based on excitation-emission fluorescence spectroscopy combined with parallel factor analysis (PARAFAC) and may constitute an alternative to fixed wavelength fluorescence and synchronous fluorescence spectroscopy (M). PARAFAC was applied to excitation-emission matrices (EEMs) of bile samples of shorthorn sculpins and European eels collected in Greenland and Denmark. The EEMs were decomposed into a four-factor PARAFAC model. The comparison of the PARAFAC factors with the EEMs of PAC metabolites and amino acids suggests that two factors are related to PAC metabolites and two correspond to fluorescent residues of tryptophan and tyrosine in bile proteins. A new standardization procedure based on the mean of the scores for the biological factors was used to correct for feeding status and sample dilution and, upon such normalization, the score plots of PARAFAC factors showed a clear distinction between exposed and nonexposed fish. A good correlation was found between the factor scores and 1-hydroxypyrene equivalents determined by SFS for high contamination levels, whereas the sensitivity was better for the EEM method

    AB - Polycyclic aromatic compound (PAC) metabolites in fish bile can be used as biomarkers for recent environmental exposure to PACs. Here, a novel method for rapid screening of nonhydrolyzed fish bile is presented. The method is based on excitation-emission fluorescence spectroscopy combined with parallel factor analysis (PARAFAC) and may constitute an alternative to fixed wavelength fluorescence and synchronous fluorescence spectroscopy (M). PARAFAC was applied to excitation-emission matrices (EEMs) of bile samples of shorthorn sculpins and European eels collected in Greenland and Denmark. The EEMs were decomposed into a four-factor PARAFAC model. The comparison of the PARAFAC factors with the EEMs of PAC metabolites and amino acids suggests that two factors are related to PAC metabolites and two correspond to fluorescent residues of tryptophan and tyrosine in bile proteins. A new standardization procedure based on the mean of the scores for the biological factors was used to correct for feeding status and sample dilution and, upon such normalization, the score plots of PARAFAC factors showed a clear distinction between exposed and nonexposed fish. A good correlation was found between the factor scores and 1-hydroxypyrene equivalents determined by SFS for high contamination levels, whereas the sensitivity was better for the EEM method

    U2 - 10.1021/es900187x

    DO - 10.1021/es900187x

    M3 - Journal article

    VL - 43

    SP - 4439

    EP - 4445

    JO - Environmental Science & Technology (Washington)

    JF - Environmental Science & Technology (Washington)

    SN - 0013-936X

    IS - 12

    ER -