Paper-based sensors for rapid detection of virulence factor produced by Pseudomonas aeruginosa

AlZahraa Alatraktchi, jafar Safaa Noori, Georgi Plamenov Tanev, John Mortensen, Maria Dimaki, Helle Krogh Johansen, Jan Madsen, Søren Molin, Winnie Edith Svendsen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Pyocyanin is a toxin produced by Pseudomonas aeruginosa. Here we describe a novel paper-based electrochemical sensor for pyocyanin detection, manufactured with a simple and inexpensive approach based on electrode printing on paper. The resulting sensors constitute an effective electrochemical method to quantify pyocyanin in bacterial cultures without the conventional time consuming pretreatment of the samples. The electrochemical properties of the paper-based sensors were evaluated by ferri/ferrocyanide as a redox mediator, and showed reliable sensing performance. The paper-based sensors readily allow for the determination of pyocyanin in bacterial cultures with high reproducibility, achieving a limit of detection of 95 nM and a sensitivity of 4.30 μA/μM in standard culture media. Compared to the similar commercial ceramic based sensors, it is a 2.3-fold enhanced performance.

The simple in-house fabrication of sensors for pyocyanin quantification allows researchers to understand in vitro adaptation of P. aeruginosa infections via rapid screenings of bacterial cultures that otherwise are expensive and time-consuming.
OriginalsprogEngelsk
Artikelnummere0194157/1
TidsskriftP L o S One
Vol/bind13
Udgave nummer3
Antal sider9
ISSN1932-6203
DOI
StatusUdgivet - 22 mar. 2018

Citer dette

Alatraktchi, AlZahraa ; Safaa Noori, jafar ; Tanev, Georgi Plamenov ; Mortensen, John ; Dimaki, Maria ; Johansen, Helle Krogh ; Madsen, Jan ; Molin, Søren ; Svendsen, Winnie Edith. / Paper-based sensors for rapid detection of virulence factor produced by Pseudomonas aeruginosa. I: P L o S One. 2018 ; Bind 13, Nr. 3.
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title = "Paper-based sensors for rapid detection of virulence factor produced by Pseudomonas aeruginosa",
abstract = "Pyocyanin is a toxin produced by Pseudomonas aeruginosa. Here we describe a novel paper-based electrochemical sensor for pyocyanin detection, manufactured with a simple and inexpensive approach based on electrode printing on paper. The resulting sensors constitute an effective electrochemical method to quantify pyocyanin in bacterial cultures without the conventional time consuming pretreatment of the samples. The electrochemical properties of the paper-based sensors were evaluated by ferri/ferrocyanide as a redox mediator, and showed reliable sensing performance. The paper-based sensors readily allow for the determination of pyocyanin in bacterial cultures with high reproducibility, achieving a limit of detection of 95 nM and a sensitivity of 4.30 μA/μM in standard culture media. Compared to the similar commercial ceramic based sensors, it is a 2.3-fold enhanced performance.The simple in-house fabrication of sensors for pyocyanin quantification allows researchers to understand in vitro adaptation of P. aeruginosa infections via rapid screenings of bacterial cultures that otherwise are expensive and time-consuming.",
author = "AlZahraa Alatraktchi and {Safaa Noori}, jafar and Tanev, {Georgi Plamenov} and John Mortensen and Maria Dimaki and Johansen, {Helle Krogh} and Jan Madsen and S{\o}ren Molin and Svendsen, {Winnie Edith}",
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Alatraktchi, A, Safaa Noori, J, Tanev, GP, Mortensen, J, Dimaki, M, Johansen, HK, Madsen, J, Molin, S & Svendsen, WE 2018, 'Paper-based sensors for rapid detection of virulence factor produced by Pseudomonas aeruginosa', P L o S One, bind 13, nr. 3, e0194157/1. https://doi.org/10.1371/journal.pone.0194157

Paper-based sensors for rapid detection of virulence factor produced by Pseudomonas aeruginosa. / Alatraktchi, AlZahraa; Safaa Noori, jafar; Tanev, Georgi Plamenov; Mortensen, John; Dimaki, Maria; Johansen, Helle Krogh; Madsen, Jan; Molin, Søren; Svendsen, Winnie Edith.

I: P L o S One, Bind 13, Nr. 3, e0194157/1, 22.03.2018.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Paper-based sensors for rapid detection of virulence factor produced by Pseudomonas aeruginosa

AU - Alatraktchi, AlZahraa

AU - Safaa Noori, jafar

AU - Tanev, Georgi Plamenov

AU - Mortensen, John

AU - Dimaki, Maria

AU - Johansen, Helle Krogh

AU - Madsen, Jan

AU - Molin, Søren

AU - Svendsen, Winnie Edith

PY - 2018/3/22

Y1 - 2018/3/22

N2 - Pyocyanin is a toxin produced by Pseudomonas aeruginosa. Here we describe a novel paper-based electrochemical sensor for pyocyanin detection, manufactured with a simple and inexpensive approach based on electrode printing on paper. The resulting sensors constitute an effective electrochemical method to quantify pyocyanin in bacterial cultures without the conventional time consuming pretreatment of the samples. The electrochemical properties of the paper-based sensors were evaluated by ferri/ferrocyanide as a redox mediator, and showed reliable sensing performance. The paper-based sensors readily allow for the determination of pyocyanin in bacterial cultures with high reproducibility, achieving a limit of detection of 95 nM and a sensitivity of 4.30 μA/μM in standard culture media. Compared to the similar commercial ceramic based sensors, it is a 2.3-fold enhanced performance.The simple in-house fabrication of sensors for pyocyanin quantification allows researchers to understand in vitro adaptation of P. aeruginosa infections via rapid screenings of bacterial cultures that otherwise are expensive and time-consuming.

AB - Pyocyanin is a toxin produced by Pseudomonas aeruginosa. Here we describe a novel paper-based electrochemical sensor for pyocyanin detection, manufactured with a simple and inexpensive approach based on electrode printing on paper. The resulting sensors constitute an effective electrochemical method to quantify pyocyanin in bacterial cultures without the conventional time consuming pretreatment of the samples. The electrochemical properties of the paper-based sensors were evaluated by ferri/ferrocyanide as a redox mediator, and showed reliable sensing performance. The paper-based sensors readily allow for the determination of pyocyanin in bacterial cultures with high reproducibility, achieving a limit of detection of 95 nM and a sensitivity of 4.30 μA/μM in standard culture media. Compared to the similar commercial ceramic based sensors, it is a 2.3-fold enhanced performance.The simple in-house fabrication of sensors for pyocyanin quantification allows researchers to understand in vitro adaptation of P. aeruginosa infections via rapid screenings of bacterial cultures that otherwise are expensive and time-consuming.

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